Characterization of cadmium plasma membrane transport in gills of a mangrove crab Ucides cordatus

Autor(es)

Ortega, P. ^[1] ; Custodio, M. R. ^[1] ; Zanotto, F. P. ^{[2, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Fisiol, Inst Biociencias, BR-05508900 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Escola Paulista Med, Dept Biofis, BR-04044020 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: AQUATIC TOXICOLOGY; v. 157, p. 21-29, DEC 2014.
• Citações Web of Science: 11

Resumo

Membrane pathway for intracellular cadmium (Cd2+) accumulation is not fully elucidated in many organisms and has not been studied in crab gill cells. To characterize membrane Cd2+ transport of anterior and posterior gill cells of Ucides cordatus, a hypo-hyper-regulating crab, a change in intracellular Cd2+ concentration under various experimental conditions was examined by using FluoZin, a fluorescent probe. The membrane Cd2+ transport was estimated by the augmentation of FluoZin fluorescence induced by extracellular application of CdCl2 and different inhibitors. Addition of extracellular calcium (Cd2+) to the cells affected little the fluorescence of FluoZin, confirming that Cd2+ was the main ion increasing intracellular fluorescence. Cd2+ channels blockers (nimodipine and verapamil) decreased Cd2+ influx as well as vanadate, a Cd2+-ATPase blocker. Chelating intracellular Cd2+ (BAPTA) decreased Cd2+ influx in gill cells, while increasing intracellular Cd2+ (caffeine) augmented Cd influx. Cd2+ and ATP added at different temporal conditions were not effective at increasing intracellular Cd2+ accumulation. Ouabain (Na+/K+-ATPase inhibitor) increased Cd2+ influx probably through a change in intracellular Na and/or a change in cell membrane potential. Routes of Cd2+ influx, a non-essential metal, through the gill cell plasma membrane of crabs are suggested. (C) 2014 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 09/15546-3 - Caracterização do transporte de cádmio em interação com o cálcio em células epiteliais de Ucides cordatus, um caranguejo de mangue
• Beneficiário: Flavia Pinheiro Zanotto
• Modalidade de apoio: Auxílio à Pesquisa - Regular