Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Characterization of cadmium plasma membrane transport in gills of a mangrove crab Ucides cordatus

Full text
Author(s):
Ortega, P. [1] ; Custodio, M. R. [1] ; Zanotto, F. P. [2, 1]
Total Authors: 3
Affiliation:
[1] Univ Sao Paulo, Dept Fisiol, Inst Biociencias, BR-05508900 Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Escola Paulista Med, Dept Biofis, BR-04044020 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: AQUATIC TOXICOLOGY; v. 157, p. 21-29, DEC 2014.
Web of Science Citations: 11
Abstract

Membrane pathway for intracellular cadmium (Cd2+) accumulation is not fully elucidated in many organisms and has not been studied in crab gill cells. To characterize membrane Cd2+ transport of anterior and posterior gill cells of Ucides cordatus, a hypo-hyper-regulating crab, a change in intracellular Cd2+ concentration under various experimental conditions was examined by using FluoZin, a fluorescent probe. The membrane Cd2+ transport was estimated by the augmentation of FluoZin fluorescence induced by extracellular application of CdCl2 and different inhibitors. Addition of extracellular calcium (Cd2+) to the cells affected little the fluorescence of FluoZin, confirming that Cd2+ was the main ion increasing intracellular fluorescence. Cd2+ channels blockers (nimodipine and verapamil) decreased Cd2+ influx as well as vanadate, a Cd2+-ATPase blocker. Chelating intracellular Cd2+ (BAPTA) decreased Cd2+ influx in gill cells, while increasing intracellular Cd2+ (caffeine) augmented Cd influx. Cd2+ and ATP added at different temporal conditions were not effective at increasing intracellular Cd2+ accumulation. Ouabain (Na+/K+-ATPase inhibitor) increased Cd2+ influx probably through a change in intracellular Na and/or a change in cell membrane potential. Routes of Cd2+ influx, a non-essential metal, through the gill cell plasma membrane of crabs are suggested. (C) 2014 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 09/15546-3 - Characterization of cadmium transport and its interaction with calcium on epithelial cells of Ucides cordatus, a mangrove crab
Grantee:Flavia Pinheiro Zanotto
Support Opportunities: Regular Research Grants