This research project to Bolsa Estágio de Pesquisa no Exterior (BEPE) aims to complement the main Post Doctorate project in progress. It would be conducted at Institute of Marine Sciences of Andalusia (ICMAN-CSIC) under the supervision of Dr. Julián Blasco. Briefly, this proposal is focused on adapt and employ new methodologies regarding subcellular metal partitioning (SMP) and specific biomarkers of defense and effects (Metallothioneins [MTs] and Aminolevulinic Acid Dehydratase [ALA-D], respectively) in Ucides cordatus tissues (hepatopancreas and anterior-posterior gills) during long-term exposure to non-essential metals (cadmium and lead) via water and food. Understanding process of metal detoxification and effects through proteins (MTs), enzymatic activity (ALA-D) and storage in metabolically inactive forms and their metabolically active concentration within an organism (SMP) may considerably improve the ability to predict the toxicity of metals and, obviously, different biological tolerances may be demonstrated. Therefore, the main objective of the study at ICMAN-CSIC is to adapt and to employ new protocols for quantifying SMP, MTs and ALA-D in Ucides cordatus tissues after three different exposure experiments (Exp.) conducted at UNIFESP: non-essential metals via water (Cd [Exp.1] and Pb [Exp.2]) and food contaminated (Rhizophora mangle leaves [Exp. 3]; the main source of food to this crustacean). It is expected that these data will help to clarify detoxification process, sublethal damages and biological tolerances addressed in the main project. The new adapted protocols will improve the reliability and consistency of analysis in this sentinel species, which could be employed in future environmental and ecological studies. In addition, our study will provide reliable information for the proper management of mangrove ecosystems.
News published in Agência FAPESP Newsletter about the scholarship: