Pulsed direct and constant direct currents in the pilocarpine iontophoresis sweat chloride test

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Souza Gomez, Carla Cristina ^{[1, 2]} ; Servidoni, Maria de Fatima ^{[1, 2, 3]} ; de Lima Marson, Fernando Augusto ^{[1, 2, 4]} ; Coelho Canavezi, Paulo Jose ^[5] ; Vinagre, Adriana Mendes ^{[1, 2]} ; Costa, Eduardo Tavares ^{[5, 6]} ; Ribeiro, Antonio Fernando ^{[1, 2]} ; Goncalves de Oliveira Ribeiro, Maria Angela ^[2] ; Dalbo Contrera Toro, Adyleia Aparecida ^{[1, 2]} ; Pavan, Celia Regina ^[3] ; Sa dos Santos Rondon, Michelle Vivine ^[3] ; Silva Lorena, Sonia Leticia ^[3] ; Vieria Junior, Francisco Ubaldi ^{[5, 7]} ; Ribeiro, Jose Dirceu ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Fac Med Sci, Dept Pediat, BR-13083887 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Fac Med Sci, Ctr Res Pediat CIPED, BR-13083887 Campinas, SP - Brazil

^[3] State Univ Campinas Unicamp, Gastroctr, Endoscopy Unit, BR-13083872 Campinas, SP - Brazil

^[4] Univ Estadual Campinas, Fac Med Sci, Dept Med Genet, BR-13083887 Campinas, SP - Brazil

^[5] Univ Estadual Campinas, Ctr Biomed Engn CEB, BR-13083970 Campinas, SP - Brazil

^[6] Univ Estadual Campinas, Fac Elect & Comp Engn, Dept Biomed Engn, BR-13083881 Campinas, SP - Brazil

^[7] Fed Inst Educ Sci & Technol Sao Paulo IFSP, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BMC PULMONARY MEDICINE; v. 14, DEC 13 2014.
• Citações Web of Science: 3

Resumo

Background: The classic sweat test (CST) is the golden standard for cystic fibrosis (CF) diagnosis. Then, our aim was compare the production and volume of sweat, and side effects caused by pulsed direct current (PDC) and constant direct current (CDC). To determine the optimal stimulation time (ST) for the sweat collection. To verify the PDC as CF diagnosis option. Methods: Prospective study with cross-sectional experimental intervention. Experiment 1 (right arm): PDC and CDC. ST at 10 min and sweat collected at 30 min. Currents of 0.5; 0.75; 1.0 and 1.5 mA and frequencies of 0, 200, 1,000 and 5,000 Hz applied. Experiment 2 (left arm): current of 1.0 mA, ST at 5 and 10 min and sweat collected at 15 and 30 min with frequencies of 0; 200; 1,000 and 5,000 Hz applied Experiments 1 and 2 were performed with current density (CD) from 0.07 to 0.21 mA/cm(2). Experiment 3: PDC was used in typical CF patients with two CFTR mutations screened and or with CF diagnosis by rectal biopsy and patients with atypical CF. Results: 48 subjects (79.16% female) with average of 29.54 +/- 8.87 years old were enrolled. There was no statistical difference between the interaction of frequency and current in the sweat weight (p = 0.7488). Individually, positive association was achieved between weight sweat and stimulation frequency (p = 0.0088); and current (p = 0.0025). The sweat production was higher for 10 min of stimulation (p = 0.0023). The sweat collection was better for 30 min (p = 0.0019). The skin impedance was not influenced by ST and sweat collection (p > 0.05). The current frequency was inversely associated with the skin impedance (p < 0.0001). The skin temperature measured before stimulation was higher than after (p < 0.0001). In Experiment 3 (29 subjects) the PDC showed better kappa index compared to CDC (0.9218 versus 0.5205, respectively). Conclusions: The performance of the CST with CDC and PDC with CD of 0.14 to 0.21 mA/cm(2) showed efficacy in steps of stimulation and collection of sweat, without side effects. The optimal stimulation time and sweat collection were, respectively, 10 and 30 min. (AU)

• Processo FAPESP: 11/12939-4 - Associação entre polimorfismos de genes moduladores em crianças e adolescentes com asma alérgica e não alérgica: leve, moderada e grave
• Beneficiário: Fernando Augusto de Lima Marson
• Linha de fomento: Bolsas no Brasil - Doutorado