Rosmarinic acid attenuates hepatic ischemia and reperfusion injury in rats

Autor(es)

Ramalho, Leandra Naira Z. ^[1] ; Pasta, Angelo Augusto C. ^[1] ; Terra, Vania Aparecida ^[2] ; Augusto, Marlei Josiele ^[1] ; Sanches, Sheila Cristina ^[1] ; Souza-Neto, Fernando P. ^[3] ; Cecchini, Rubens ^[3] ; Gulin, Francine ^[1] ; Ramalho, Fernando Silva ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Pathol, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Pontificia Univ Catolica Parana, Londrina, PR - Brazil

^[3] Univ Estadual Londrina, Dept Pathol Sci, Londrina, PR - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Food and Chemical Toxicology; v. 74, p. 270-278, DEC 2014.
• Citações Web of Science: 18

Resumo

Rosmarinic acid (RosmA) demonstrates antioxidant and anti-inflammatory properties. We investigated the effect of RosmA on liver ischemia/reperfusion injury. Rats were submitted to 60 min of ischemia plus saline or RosmA treatment (150 mg/kg BW intraperitoneally) followed by 6 h of reperfusion. Hepatocellular injury was evaluated according to aminotransferase activity and histological damage. Hepatic neutrophil accumulation was also evaluated. Oxidative/nitrosative stress was estimated by measuring the reduced glutathione, lipid hydroperoxide and nitrotyrosine levels. Endothelial and inducible nitric oxide synthase (eNOS/iNOS) and nitric oxide (NO) were assessed with immunoblotting and chemiluminescence assays. Hepatic tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta mRNA were assessed using real-time PCR, and nuclear factor-kappaB (NF-kappa B) activation was estimated by immunostaining. RosmA treatment reduced hepatocellular damage, neutrophil infiltration and all oxidative/nitrosative stress parameters. RosmA decreased the liver content of eNOS/iNOS and NO, attenuated NF-kappa B activation, and down-regulated TNF-alpha and interleukin-1beta gene expression. These data indicate that RosmA exerts anti-inflammatory and antioxidant effects in the ischernic liver, thereby protecting hepatocytes against ischemia/reperfusion injury. The mechanisms underlying these effects may be related to the inhibitory potential of RosmA on the NF-kappa B signaling pathway and the reduction of iNOS and eNOS expressions and NO levels, in addition to its natural antioxidant capability. (C) 2014 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 08/01296-2 - Efeito do éster fenetil do ácido cafeico na lesão hepática por isquemia e reperfusão após transplante hepático experimental
• Beneficiário: Sérgio Zucoloto
• Modalidade de apoio: Auxílio à Pesquisa - Regular