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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Rosmarinic acid attenuates hepatic ischemia and reperfusion injury in rats

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Author(s):
Ramalho, Leandra Naira Z. [1] ; Pasta, Angelo Augusto C. [1] ; Terra, Vania Aparecida [2] ; Augusto, Marlei Josiele [1] ; Sanches, Sheila Cristina [1] ; Souza-Neto, Fernando P. [3] ; Cecchini, Rubens [3] ; Gulin, Francine [1] ; Ramalho, Fernando Silva [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Pathol, BR-14049900 Ribeirao Preto, SP - Brazil
[2] Pontificia Univ Catolica Parana, Londrina, PR - Brazil
[3] Univ Estadual Londrina, Dept Pathol Sci, Londrina, PR - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Food and Chemical Toxicology; v. 74, p. 270-278, DEC 2014.
Web of Science Citations: 18
Abstract

Rosmarinic acid (RosmA) demonstrates antioxidant and anti-inflammatory properties. We investigated the effect of RosmA on liver ischemia/reperfusion injury. Rats were submitted to 60 min of ischemia plus saline or RosmA treatment (150 mg/kg BW intraperitoneally) followed by 6 h of reperfusion. Hepatocellular injury was evaluated according to aminotransferase activity and histological damage. Hepatic neutrophil accumulation was also evaluated. Oxidative/nitrosative stress was estimated by measuring the reduced glutathione, lipid hydroperoxide and nitrotyrosine levels. Endothelial and inducible nitric oxide synthase (eNOS/iNOS) and nitric oxide (NO) were assessed with immunoblotting and chemiluminescence assays. Hepatic tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta mRNA were assessed using real-time PCR, and nuclear factor-kappaB (NF-kappa B) activation was estimated by immunostaining. RosmA treatment reduced hepatocellular damage, neutrophil infiltration and all oxidative/nitrosative stress parameters. RosmA decreased the liver content of eNOS/iNOS and NO, attenuated NF-kappa B activation, and down-regulated TNF-alpha and interleukin-1beta gene expression. These data indicate that RosmA exerts anti-inflammatory and antioxidant effects in the ischernic liver, thereby protecting hepatocytes against ischemia/reperfusion injury. The mechanisms underlying these effects may be related to the inhibitory potential of RosmA on the NF-kappa B signaling pathway and the reduction of iNOS and eNOS expressions and NO levels, in addition to its natural antioxidant capability. (C) 2014 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 08/01296-2 - Effect of caffeic acid phenethyl ester on hepatic ischemia and reperfusion injury after experimental liver transplantation
Grantee:Sérgio Zucoloto
Support Opportunities: Regular Research Grants