Simultaneous quantification of phenolic compounds in buriti fruit (Mauritia flexuosa L.f.) by ultra-high performance liquid chromatography coupled to tandem mass spectrometry

Autor(es)

Bataglion, Giovana A. ^[1] ; da Silva, Felipe M. A. ^[2] ; Eberlin, Marcos N. ^[1] ; Koolen, Hector H. F. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Chem, ThoMSon Mass Spectrometry Lab, BR-13083970 Campinas, SP - Brazil

^[2] Univ Fed Amazonas, Dept Chem, BR-69077000 Manaus, Amazonas - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Food Research International; v. 66, p. 396-400, DEC 2014.
• Citações Web of Science: 23

Resumo

A novel analytical approach based on UHPLC-ESI(-)-MS/MS has been performed and evaluated for the identification and quantification of phenolic compounds (phenolic acids and flavonoids/catechins) in buriti (Mauritia flexuosa L.f.) fruit pulp. Due to the selectivity provided by MMR monitoring, UHPLC separation required only 10 min per analysis. Linearity (0.994 < r(2) < 0.999), limit of detection (8.39-109.80 pg mL(-1)) and limit of quantification (25.42-332.90 pg mL(-1)), inter- and intraday accuracies (> 80%) and precisions (CV < 20%), and recovery rate (< 80%) show that the developed method is reliable to analyze phenolic compounds in fruit extracts. Among the thirteen phenolic compounds analyzed by proposed method, buriti fruit pulp extract showed 6 phenolic acids such as p-coumaric acid, ferulic acid, caffeic add, protocatechuic acid, chlorogenic acid and quinic acid and 7 flavonoids such as (+)-catechin, (-)-epicatechin, apigenin, luteolin, myricetin, kaempferol and quercetin. As a conclusion, the method was successfully developed and applied representing a contribution to the chemical characterization and quantification of phenolic compounds from buriti fruits which have acknowledged antioxidant and antimicrobial activities. (C) 2014 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 12/21395-0 - Caracterização molecular da matéria orgânica sedimentar do Sistema Estuarino-Lagunar de Cananéia-Iguape por espectrometria de massas de ressonânica ciclotrônica de íons com transformada de Fourier
• Beneficiário: Giovana Anceski Bataglion
• Modalidade de apoio: Bolsas no Brasil - Doutorado