Frequencies of virulence genes and pulse field gel electrophoresis fingerprints in Escherichia coli isolates from canine pyometra

Autor(es)

Maluta, Renato P. ^[1] ; Borges, Clarissa A. ^[1] ; Beraldo, Livia G. ^[1] ; Cardozo, Marita V. ^[1] ; Voorwald, Fabiana A. ^[2] ; Santana, Andre M. ^[2] ; Rigobelo, Everlon C. ^[3] ; Toniollo, Gilson H. ^[2] ; Avila, Fernando A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Fac Ciencias Agr & Vet Jaboticabal, Dept Patol Vet, Programa Pos Grad Microbiol Agr, Sao Paulo - Brazil

^[2] Univ Estadual Paulista, Fac Ciencias Agr & Vet Jaboticabal, Dept Clin & Cirurgia Vet, Sao Paulo - Brazil

^[3] Univ Estadual Paulista, Fac Ciencias Agr & Vet Jaboticabal, Dept Prod Vegetal, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: VETERINARY JOURNAL; v. 202, n. 2, p. 393-395, NOV 2014.
• Citações Web of Science: 4

Resumo

Escherichia coli is the most common bacterial agent isolated from canine pyometra. The frequencies of 24 virulence genes and pulsed field gel electrophoresis (PFGE) profiles were determined for 23 E. coli isolates from cases of canine pyometra in Brazil. The frequencies of virulence genes were 91.3% fimH, 91.3% irp-2, 82.6% fyuA, 56.5% iroN, 47.8% traT, 39.1% usp, 34.8% sfaD/E, 34.8% tsh, 30.4% papC, 30.4% hlyA, 26.1% papGIII, 26.1% cnf-1, 21.7% papE/F, 21.7% iss, 17.4% iutA, 17.4% ompT, 17.4% cvaC, 17.4% hlyF, 17.4% iucD, 13.0% iucC, 13.0% astA, 4.3% papGII, 0% afaB/C and 0% papGI. The high frequency of yersiniabactin (fyuA and irp2) and salmochelin (iroN) genes suggests that iron uptake systems might be important in the pathogenesis of canine pyometra. PFGE profiles of 19 isolates were heterogeneous, confirming that E. coli isolates from canine pyometra are unlikely to be epidemic clones. (C) 2014 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 08/00417-0 - Caracterização bioquímica, sorológica e genotípica de cepas de Escherichia coli isoladas de água, fezes e carcaças de ovinos criados no estado de São Paulo, Brasil
• Beneficiário: Renato Pariz Maluta
• Modalidade de apoio: Bolsas no Brasil - Doutorado