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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Frequencies of virulence genes and pulse field gel electrophoresis fingerprints in Escherichia coli isolates from canine pyometra

Full text
Author(s):
Maluta, Renato P. [1] ; Borges, Clarissa A. [1] ; Beraldo, Livia G. [1] ; Cardozo, Marita V. [1] ; Voorwald, Fabiana A. [2] ; Santana, Andre M. [2] ; Rigobelo, Everlon C. [3] ; Toniollo, Gilson H. [2] ; Avila, Fernando A. [1]
Total Authors: 9
Affiliation:
[1] Univ Estadual Paulista, Fac Ciencias Agr & Vet Jaboticabal, Dept Patol Vet, Programa Pos Grad Microbiol Agr, Sao Paulo - Brazil
[2] Univ Estadual Paulista, Fac Ciencias Agr & Vet Jaboticabal, Dept Clin & Cirurgia Vet, Sao Paulo - Brazil
[3] Univ Estadual Paulista, Fac Ciencias Agr & Vet Jaboticabal, Dept Prod Vegetal, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: VETERINARY JOURNAL; v. 202, n. 2, p. 393-395, NOV 2014.
Web of Science Citations: 4
Abstract

Escherichia coli is the most common bacterial agent isolated from canine pyometra. The frequencies of 24 virulence genes and pulsed field gel electrophoresis (PFGE) profiles were determined for 23 E. coli isolates from cases of canine pyometra in Brazil. The frequencies of virulence genes were 91.3% fimH, 91.3% irp-2, 82.6% fyuA, 56.5% iroN, 47.8% traT, 39.1% usp, 34.8% sfaD/E, 34.8% tsh, 30.4% papC, 30.4% hlyA, 26.1% papGIII, 26.1% cnf-1, 21.7% papE/F, 21.7% iss, 17.4% iutA, 17.4% ompT, 17.4% cvaC, 17.4% hlyF, 17.4% iucD, 13.0% iucC, 13.0% astA, 4.3% papGII, 0% afaB/C and 0% papGI. The high frequency of yersiniabactin (fyuA and irp2) and salmochelin (iroN) genes suggests that iron uptake systems might be important in the pathogenesis of canine pyometra. PFGE profiles of 19 isolates were heterogeneous, confirming that E. coli isolates from canine pyometra are unlikely to be epidemic clones. (C) 2014 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 08/00417-0 - Biochemical, serological and genotypic characterization of Escherichia coli strains isolated from water, feces and carcass from sheep raised in São Paulo State, Brazil
Grantee:Renato Pariz Maluta
Support Opportunities: Scholarships in Brazil - Doctorate