Antifungals and acetylcholinesterase inhibitors from the stem bark of Croton heliotropiifolius

Autor(es):Mostrar menos -

Queiroz, M. M. F. ^{[1, 2]} ; Queiroz, E. F. ^[2] ; Zeraik, M. L. ^[1] ; Marti, G. ^[1] ; Favre-Godal, Q. ^[2] ; Simoes-Pires, C. ^[2] ; Marcourt, L. ^[2] ; Carrupt, P. A. ^[2] ; Cuendet, M. ^[2] ; Paulo, M. Q. ^[3] ; Bolzani, V. S. ^[1] ; Wolfender, J. L. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Inst Quim, NuBBE, Nucleo Bioensaios Biossintese & Ecofisiol Prod Na, Sao Paulo - Brazil

^[2] Univ Lausanne, Univ Geneva, Sch Pharmaceut Sci, CH-1211 Geneva 4 - Switzerland

^[3] Univ Fed Paraiba, Lab Quim Prod Nat, UFPB, BR-58000 Joao Pessoa, Paraiba - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PHYTOCHEMISTRY LETTERS; v. 10, p. LXXXVIII-xciii, DEC 2014.
• Citações Web of Science: 10

Resumo

The ethanolic extract of the stem bark of Croton heliotropiifolius Kunth (Euphorbiaceae) showed significant in vitro inhibition of acetylcholinesterase using a dilution spectrophotometric assay and antifungal activity against Candida albicans with a thin layer chromatography (TLC) bioautographic assay. In order to isolate the active compounds, bioassay-guided fractionation was undertaken using HPLC to localize the active compounds. Different zones of the HPLC-UV chromatogram were linked to acetylcholinesterase inhibition or to antifungal activities. In parallel to this HPLC-based activity profiling, HPLC-PDA-ESI-MS and HPLC-TOF-HRMS were used for the early identification of some of the compounds present. The targeted isolation of the active compounds was performed by medium pressure liquid chromatography (MPLC-UV) and further semi-preparative HPLC. Using this approach, nine compounds were isolated, one of them being a new indole alkaloid derivative. The structures of the isolated compounds were elucidated by spectroscopic methods including UV, NMR, MS and HRMS. (C) 2014 Phytochemical Society of Europe. Published by Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 10/09780-0 - Uso da espectrometria de massas e da ressonância magnética nuclear para avaliação metabolômica & Identificação de protótipos inibidores de AChE em Banisteriopsis variabilis(B. Gates)- Malpighiaceae
• Beneficiário: Marcos Marçal Ferreira Queiroz
• Modalidade de apoio: Bolsas no Brasil - Doutorado