Comparative study of the bioconversion process using R-(+)- and S-(-)-limonene as substrates for Fusarium oxysporum 152B

Autor(es)

Molina, Gustavo ^{[1, 2]} ; Bution, Murillo L. ^[1] ; Bicas, Juliano L. ^[1] ; Dolder, Mary Anne Heidi ^[3] ; Pastore, Glaucia M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Lab Bioflavors, Dept Food Sci, Sch Food Engn, BR-13083862 Sao Paulo - Brazil

^[2] Univ Fed Vales Jequitinhonha & Mucuri, Inst Sci & Technol, Diamantina, MG - Brazil

^[3] Univ Estadual Campinas, Inst Biol, Dept Struct & Funct Biol, BR-13083862 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Food Chemistry; v. 174, p. 606-613, MAY 1 2015.
• Citações Web of Science: 11

Resumo

This study compared the bioconversion process of S-(-)-limonene into limonene-1,2-diol with the already established biotransformation of R-(+)-limonene into alpha-terpineol using the same biocatalyst in both processes, Fusarium oxysporum 152B. The bioconversion of the S-(-)-isomer was tested on cell permeabilisation under anaerobic conditions and using a biphasic system. When submitted to permeabilisation trials, this biocatalyst has shown a relatively high resistance; still, no production of limonene-1,2-diol and a loss of activity of the biocatalyst were observed after intense cell treatment, indicating a complete loss of cell viability. Furthermore, the results showed that this process can be characterised as an aerobic system that was catalysed by limonene-1,2-epoxide hydrolase, had an intracellular nature and was cofactor-dependent because the final product was not detected by an anaerobic process. Finally, this is the first report to characterise the bioconversion of R-(+)- and S-(-)-limonene by cellular detoxification using ultra-structural analysis. (C) 2014 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 11/50687-7 - Produção biotecnológica de compostos de aroma por biotransformação de terpenos
• Beneficiário: Glaucia Maria Pastore
• Modalidade de apoio: Auxílio à Pesquisa - Regular