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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Comparative study of the bioconversion process using R-(+)- and S-(-)-limonene as substrates for Fusarium oxysporum 152B

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Author(s):
Molina, Gustavo [1, 2] ; Bution, Murillo L. [1] ; Bicas, Juliano L. [1] ; Dolder, Mary Anne Heidi [3] ; Pastore, Glaucia M. [1]
Total Authors: 5
Affiliation:
[1] Univ Estadual Campinas, Lab Bioflavors, Dept Food Sci, Sch Food Engn, BR-13083862 Sao Paulo - Brazil
[2] Univ Fed Vales Jequitinhonha & Mucuri, Inst Sci & Technol, Diamantina, MG - Brazil
[3] Univ Estadual Campinas, Inst Biol, Dept Struct & Funct Biol, BR-13083862 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Food Chemistry; v. 174, p. 606-613, MAY 1 2015.
Web of Science Citations: 11
Abstract

This study compared the bioconversion process of S-(-)-limonene into limonene-1,2-diol with the already established biotransformation of R-(+)-limonene into alpha-terpineol using the same biocatalyst in both processes, Fusarium oxysporum 152B. The bioconversion of the S-(-)-isomer was tested on cell permeabilisation under anaerobic conditions and using a biphasic system. When submitted to permeabilisation trials, this biocatalyst has shown a relatively high resistance; still, no production of limonene-1,2-diol and a loss of activity of the biocatalyst were observed after intense cell treatment, indicating a complete loss of cell viability. Furthermore, the results showed that this process can be characterised as an aerobic system that was catalysed by limonene-1,2-epoxide hydrolase, had an intracellular nature and was cofactor-dependent because the final product was not detected by an anaerobic process. Finally, this is the first report to characterise the bioconversion of R-(+)- and S-(-)-limonene by cellular detoxification using ultra-structural analysis. (C) 2014 Elsevier Ltd. All rights reserved. (AU)