| Full text | |
| Author(s): |
Molina, Gustavo
[1, 2]
;
Bution, Murillo L.
[1]
;
Bicas, Juliano L.
[1]
;
Dolder, Mary Anne Heidi
[3]
;
Pastore, Glaucia M.
[1]
Total Authors: 5
|
| Affiliation: | [1] Univ Estadual Campinas, Lab Bioflavors, Dept Food Sci, Sch Food Engn, BR-13083862 Sao Paulo - Brazil
[2] Univ Fed Vales Jequitinhonha & Mucuri, Inst Sci & Technol, Diamantina, MG - Brazil
[3] Univ Estadual Campinas, Inst Biol, Dept Struct & Funct Biol, BR-13083862 Sao Paulo - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Food Chemistry; v. 174, p. 606-613, MAY 1 2015. |
| Web of Science Citations: | 11 |
| Abstract | |
This study compared the bioconversion process of S-(-)-limonene into limonene-1,2-diol with the already established biotransformation of R-(+)-limonene into alpha-terpineol using the same biocatalyst in both processes, Fusarium oxysporum 152B. The bioconversion of the S-(-)-isomer was tested on cell permeabilisation under anaerobic conditions and using a biphasic system. When submitted to permeabilisation trials, this biocatalyst has shown a relatively high resistance; still, no production of limonene-1,2-diol and a loss of activity of the biocatalyst were observed after intense cell treatment, indicating a complete loss of cell viability. Furthermore, the results showed that this process can be characterised as an aerobic system that was catalysed by limonene-1,2-epoxide hydrolase, had an intracellular nature and was cofactor-dependent because the final product was not detected by an anaerobic process. Finally, this is the first report to characterise the bioconversion of R-(+)- and S-(-)-limonene by cellular detoxification using ultra-structural analysis. (C) 2014 Elsevier Ltd. All rights reserved. (AU) | |