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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Omega-3 Supplementation Improves Pancreatic Islet Redox Status In Vivo and In Vitro Studies

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Autor(es):
Lucena, Camila F. [1] ; Roma, Leticia P. [1] ; Graciano, Maria Fernanda R. [2] ; Veras, Katherine [1] ; Simoes, Daniel [1] ; Curi, Rui [1] ; Carpinelli, Angelo R. [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-05508000 Sao Paulo - Brazil
[2] Univ Londrina, Dept Physiol Sci, Londrina, Parana - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: PANCREAS; v. 44, n. 2, p. 287-295, MAR 2015.
Citações Web of Science: 6
Resumo

Objectives: The aim of the study was to evaluate the potential changes induced by fish oil (FO) supplementation on the redox status of pancreatic islets from healthy rats. To test whether these effects were due to eicosapentaenoic acid and docosahexaenoic acid (omega-3), in vitro experiments were performed. Methods: Rats were supplemented with FO, and pancreatic islets were obtained. Islets were also treated in vitro with palmitate (P) or eicosapentaenoic acid + docosahexaenoic acid (omega-3). Insulin secretion (GSIS), glucose oxidation, protein expression, and superoxide content were analyzed. Results: The FO group showed a reduction in superoxide content. Moreover, FO reduced the expression of NAD(P) H oxidase subunits and increased superoxide dismutase, without altering beta-cell function. Palmitate increased beta-cell reactive oxygen species (ROS) production, apoptosis, and impaired GSIS. Under these conditions, omega-3 triggered a parallel reduction in ROS production and beta-cell apoptosis induced by P and protected against the impairment in GSIS. There was no difference in mitochondrial ROS production. Conclusions: Our results show that omega-3 protect pancreatic islets from alterations induced by P. In vivo FO supplementation modulates the redox state of pancreatic beta-cell. Considering that in vitro effects do not involve mitochondrial superoxide production, we can speculate that this protection might involve NAD(P) H oxidase activity. (AU)

Processo FAPESP: 12/16374-4 - Possíveis efeitos protetores de epa e dha contra apoptose induzida por palmitato em ilhotas pancreáticas em cultura
Beneficiário:Camila Ferraz Lucena Monaco
Linha de fomento: Bolsas no Brasil - Doutorado