Screening of thermotolerant and thermophilic fungi aiming beta-xylosidase and arabinanase production

Autor(es)

Benassi, Vivian Machado ^[1] ; de Lucas, Rosymar Coutinho ^[1] ; Jorge, Joao Atilio ^[2] ; Teixeira de Moraes Polizeli, Maria de Lourdes ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Bioquim & Imunol, Fac Med Ribeirao Preto, BR-14040901 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Dept Biol, Fac Filosofia Ciencias & Letras Ribeirao Preto, BR-14040901 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Microbiology; v. 45, n. 4, p. 1459-1467, OCT-DEC 2014.
• Citações Web of Science: 4

Resumo

Plant cell wall is mainly composed by cellulose, hemicellulose and lignin. The heterogeneous structure and composition of the hemicellulose are key impediments to its depolymerization and subsequent use in fermentation processes. Thus, this study aimed to perform a screening of thermophilic and thermotolerant filamentous fungi collected from different regions of the Sao Paulo state, and analyze the production of beta-xylosidase and arabinanase at different temperatures. These enzymes are important to cell wall degradation and synthesis of end products as xylose and arabinose, respectively, which are significant sugars to fermentation and ethanol production. A total of 12 fungal species were analyzed and 9 of them grew at 45 degrees C, suggesting a thermophilic or thermotolerant character. Additionally Aspergillus thermomutatus anamorph of Neosartorya and A. parasiticus grew at 50 degrees C. Aspergillus niger and Aspergillus thermomutatus were the filamentous fungi with the most expressive production of beta-xylosidase and arabinanase, respectively. In general for most of the tested microorganisms, beta-xylosidase and arabinanase activities from mycelial extract (intracellular form) were higher in cultures grown at high temperatures (35-40 degrees C), while the correspondent extracellular activities were favorably secreted from cultures at 30 degrees C. This study contributes to catalogue isolated fungi of the state of Sao Paulo, and these findings could be promising sources for thermophilic and thermotolerant microorganisms, which are industrially important due to their enzymes. (AU)

• Processo FAPESP: 10/52322-3 - Bioprospecção de fungos filamentosos produtores de holoenzimas com aplicação em biorefinaria
• Beneficiário: Maria de Lourdes Teixeira de Moraes Polizeli
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular