Mesenchymal stem cells engrafted in a fibrin scaffold stimulate Schwann cell reactivity and axonal regeneration following sciatic nerve tubulization

Autor(es)

Cartarozzi, Luciana P. ^[1] ; Spejo, Aline B. ^[1] ; Ferreira, Jr., Rui S. ^[2] ; Barraviera, Benedito ^[2] ; Duek, Eliana ^[3] ; Carvalho, Juliana L. ^[4] ; Goes, Alfredo M. ^[4] ; Oliveira, Alexandre L. R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Dept Struct & Funct Biol, BR-13083970 Campinas, SP - Brazil

^[2] UNESP, CEVAP, BR-18610307 Botucatu, SP - Brazil

^[3] Univ Estadual Campinas, Fac Mech Engn, BR-13083860 Campinas, SP - Brazil

^[4] Univ Fed Minas Gerais, Inst Biol Sci, BR-30121970 Belo Horizonte, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brain Research Bulletin; v. 112, p. 14-24, MAR 2015.
• Citações Web of Science: 22

Resumo

The present study investigated the effectiveness of mesenchymal stem cells (MSCs) associated with a fibrin scaffold (FS) for the peripheral regenerative process after nerve tubulization. Adult female Lewis rats received a unilateral sciatic nerve transection followed by repair with a polycaprolactone (PCL)-based tubular prosthesis. Sixty days after injury, the regenerated nerves were studied by immunohistochemistry. Anti-p75NTR immunostaining was used to investigate the reactivity of the MSCs. Basal labeling, which was upregulated during the regenerative process, was detected in uninjured nerves and was significantly greater in the MSC-treated group. The presence of GFP-positive MSCs was detected in the nerves, indicating the long term survival of such cells. Moreover, there was co-localization between MSCs and BNDF immunoreactivity, showing a possible mechanism by which MSCs improve the reactivity of SCs. Myelinated axon counting and morphometric analyses showed that MSC engrafting led to a higher degree of fiber compaction combined with a trend of increased myelin sheath thickness, when compared with other groups. The functional result of MSC engrafting was that the animals showed higher motor function recovery at the seventh and eighth week after lesion. The findings herein show that MSC+FS therapy improves the nerve regeneration process by positively modulating the reactivity of SCs. (C) 2015 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 14/06892-3 - Utilização de células tronco mesenquimais na interface do sistema nervoso central e periférico: reparo de lesões proximais
• Beneficiário: Alexandre Leite Rodrigues de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 12/08101-8 - Imageamento in vivo da regeneração nervosa após reimplante de raízes motoras com selante de fibrina fluo-marcado associado com células tronco mesenquimais
• Beneficiário: Rui Seabra Ferreira Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/23236-4 - Toxinas animais nativas e recombinantes: análise funcional, estrutural e molecular
• Beneficiário: Suely Vilela
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 09/53846-9 - EMU: aquisição de cromatógrafo líquido de alta eficiência (CLAE) preparativo para purificação e isolamento de serinoproteinases para produção do selante de fibrina
• Beneficiário: Benedito Barraviera
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários