Chemotactic signal transduction and phosphate metabolism as adaptive strategies during citrus canker induction by Xanthomonas citri

Autor(es)

Moreira, Leandro Marcio ^{[1, 2]} ; Facincani, Agda Paula ^[3] ; Ferreira, Cristiano Barbalho ^[3] ; Ferreira, Rafael Marine ^[3] ; Tiraboshi Ferro, Maria Ines ^[3] ; Gozzo, Fabio Cesar ^[4] ; Franco de Oliveira, Julio Cezar ^[5] ; Ferro, Jesus Aparecido ^[3] ; Soares, Marcia Regina ^[6]

Afiliação do(s) autor(es)

^[1] Univ Fed Ouro Preto, Dept Ciencias Biol, Inst Ciencias Exatas & Biol, Ouro Preto, MG - Brazil

^[2] Univ Fed Ouro Preto, Nucleo Pesquisas Ciencias Biol NUPEB, Ouro Preto, MG - Brazil

^[3] UNESP Univ Estadual Paulista, Dept Tecnol, Fac Ciencias Agr & Vet Jaboticabal, Sao Paulo, SP - Brazil

^[4] Univ Estadual Campinas, Inst Quim, UNICAMP, Campinas, SP - Brazil

^[5] Univ Fed Sao Paulo UNIFESP, Dept Ciencias Biol, Diadema, SP - Brazil

^[6] Univ Fed Rio de Janeiro, Dept Bioquim, Inst Quim, Rio De Janeiro, RJ - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FUNCTIONAL & INTEGRATIVE GENOMICS; v. 15, n. 2, p. 197-210, MAR 2015.
• Citações Web of Science: 14

Resumo

The genome of Xanthomonas citri subsp. Citri strain 306 pathotype A (Xac) was completely sequenced more than 10 years; to date, few studies involving functional genomics Xac and its host compatible have been developed, specially related to adaptive events that allow the survival of Xac within the plant. Proteomic analysis of Xac showed that the processes of chemotactic signal transduction and phosphate metabolism are key adaptive strategies during the interaction of a pathogenic bacterium with its plant host. The results also indicate the importance of a group of proteins that may not be directly related to the classical virulence factors, but that are likely fundamental to the success of the initial stages of the infection, such as methyl-accepting chemotaxis protein (Mcp) and phosphate specific transport (Pst). Furthermore, the analysis of the mutant of the gene pstB which codifies to an ABC phosphate transporter subunit revealed a complete absence of citrus canker symptoms when inoculated in compatible hosts. We also conducted an in silico analysis which established the possible network of genes regulated by two-component systems PhoPQ and PhoBR (related to phosphate metabolism), and possible transcriptional factor binding site (TFBS) motifs of regulatory proteins PhoB and PhoP, detaching high degree of conservation of PhoB TFBS in 84 genes of Xac genome. This is the first time that chemotaxis signal transduction and phosphate metabolism were therefore indicated to be fundamental to the process of colonization of plant tissue during the induction of disease associated with Xanthomonas genus bacteria. (AU)

• Processo FAPESP: 04/02006-7 - Genoma funcional do cancro cítrico: estudo de interações patógeno-planta
• Beneficiário: Julio Cezar Franco de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores