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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Validation of methylation markers for diagnosis of oral cavity cancer

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Autor(es):
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Arantes, L. M. R. B. ; de Carvalho, A. C. ; Melendez, M. E. ; Centrone, C. C. ; Gois-Filho, J. F. ; Toporcov, T. N. ; Caly, D. N. ; Tajara, E. H. ; Goloni-Bertollo, E. M. ; Carvalho, A. L. ; GENCAPO
Número total de Autores: 11
Tipo de documento: Artigo Científico
Fonte: EUROPEAN JOURNAL OF CANCER; v. 51, n. 5, p. 632-641, MAR 2015.
Citações Web of Science: 20
Assunto(s):Neoplasias de cabeça e pescoço   Biomarcadores   Metilação de DNA
Resumo

Purpose: Activation of proto-oncogenes and inactivation of tumour suppressor genes are the major genetic alterations involved in carcinogenesis. The increase in methylation at the promoter region of a tumour suppressor gene can lead to gene inactivation, selecting cells with proliferative advantage. Thus, promoter hypermethylation is considered a marker in a variety of malignant tumours, including oral cavity. Experimental design: The methylation pattern of eight genes was evaluated in 40 oral cavity squamous cell carcinomas (OSCCs) and 40 saliva samples from healthy individuals by Q-MSP. Different combinations of genes were also assessed in order to identify gene panels that could better distinguish between OSCC and saliva samples. Results: CCNA1, DAPK, DCC and TIMP3 methylation were highly specific for being found in the OSCC samples. Moreover, the combination of these genes improved detection when compared with single markers, reaching values of 92.5% for sensitivity and specificity (when using the panel CCNA1, DCC, TIMP3). Moreover, DAPK, DCC and TIMP3 were hypermethylated in nearly 90% of clinically T1 and T2 cases. Conclusion: The pursuing of this panel of hypermethylated genes is an important tool for the detection of individuals with OSCC. Moreover, the identification of these markers in early stages of OSCC shows the feasibility of using the panel on saliva as possible biomarkers for early diagnosis. The lack of association between the methylation status of these genes and clinical characteristics shows that they are able to distinguish OSCC cases irrespective of social and clinical factors (gender, age, human papillomavirus (HPV) status, clinical stage, vascular embolisation and perineural invasion). (C) 2015 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 11/02864-7 - Alterações epigenéticas como biomarcadores em câncer de cabeça e pescoço
Beneficiário:Lidia Maria Rebolho Batista Arantes
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 10/51168-0 - Fatores ambientais, clínicos, histopatológicos e moleculares associados ao desenvolvimento e ao prognóstico de carcinomas epidermoides de cabeça e pescoço
Beneficiário:Eloiza Helena Tajara da Silva
Linha de fomento: Auxílio à Pesquisa - Temático