Separation of glycosidic catiomers by TWIM-MS using CO2 as a drift gas

Autor(es)

Bataglion, Giovana A. ^[1] ; Martins Ferreira Souza, Gustavo Henrique ^[2] ; Heerdt, Gabriel ^[3] ; Morgon, Nelson H. ^[3] ; Lisboa Dutra, Jose Diogo ^{[4, 5]} ; Freire, Ricardo Oliveira ^[4] ; Eberlin, Marcos N. ^[1] ; Tata, Alessandra ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Inst Chem, ThoMSon Mass Spectrometry Lab, BR-13084971 Campinas, SP - Brazil

^[2] Waters Corp, Applicat Lab Brazil, Sao Paulo, SP - Brazil

^[3] Univ Campinas UNICAMP, Inst Chem, Campinas, SP - Brazil

^[4] Univ Fed Sergipe, Dept Quim, Pople Computat Chem Lab, BR-49100000 Sao Cristovao, SE - Brazil

^[5] Univ Fed Pernambuco, Dept Quim Fundamental, UFPE, BR-50590470 Recife, PE - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Mass Spectrometry; v. 50, n. 2, p. 336-343, FEB 2015.
• Citações Web of Science: 11

Resumo

Traveling wave ion mobility mass spectrometry (TWIM-MS) is shown to be able to separate and characterize several isomeric forms of diterpene glycosides stevioside (Stv) and rebaudioside A (RebA) that are cationized by Na+ and K+ at different sites. Determination and characterization of these coexisting isomeric species, herein termed catiomers, arising from cationization at different and highly competitive coordinating sites, is particularly challenging for glycosides. To achieve this goal, the advantage of using CO2 as a more massive and polarizable drift gas, over N-2, was demonstrated. Post-TWIM-MS/MS experiments were used to confirm the separation. Optimization of the possible geometries and cross-sectional calculations for mobility peak assignments were also performed. Copyright (c) 2015 John Wiley \& Sons, Ltd. (AU)

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