Absence of Microsatellite Instability In Soft Tissue Sarcomas

Autor(es)

Campanella, Nathalia C. ^[1] ; Penna, Valter ^[2] ; Ribeiro, Guilherme ^[3] ; Abrahao-Machado, Lucas Faria ^{[3, 4]} ; Scapulatempo-Neto, Cristovam ^{[1, 2]} ; Reis, Rui Manuel ^{[1, 5, 6]}

Afiliação do(s) autor(es)

^[1] Barretos Canc Hosp, Mol Oncol Res Ctr, BR-14784400 Barretos, SP - Brazil

^[2] Barretos Canc Hosp, Dept Orthopedy, BR-14784400 Barretos, SP - Brazil

^[3] Barretos Canc Hosp, Dept Pathol, BR-14784400 Barretos, SP - Brazil

^[4] Barretos Canc Hosp, Dept Citogenet, BR-14784400 Barretos, SP - Brazil

^[5] Univ Minho, Sch Hlth Sci, Life & Hlth Sci Res Inst ICVS, Braga - Portugal

^[6] ICVS 3Bs PT Govt Associate Lab, Braga - Portugal

• Tipo de documento: Artigo Científico
• Fonte: PATHOBIOLOGY; v. 82, n. 1, p. 36-42, 2015.
• Citações Web of Science: 5

Resumo

Objective: Here, we analyze a series of soft tissue sarcomas (STS), which are a heterogeneous group of mesenchymal neoplasms, for the presence and frequency of microsatellite instability (MSI). MSI has been proposed to be clinically relevant for colorectal cancer, yet on STS its role is not consensual, partly due to the limited number of cases analyzed and methodology issues. Methods: The detailed evaluation of MSI in tumor samples from 71 STS patients was performed by pentaplex PCR of the MSI markers NR-27, NR-21, NR-24, BAT-25, and BAT-26, followed by capillary electrophoresis. The expression of DNA mismatch repair (MMR) proteins (MLH1, MSH2, MSH6, and PMS2) was also evaluated in suspected MSI-positive cases by immunohistochemistry. Results: The MSI analysis showed instability of one MSI marker in a total of 3 cases (4.2%). However, MMR protein expression was not affected, demonstrating that all cases were microsatellite stable. Conclusion: Our results suggest that MSI does not play a role in STS tumorigenesis. (C) 2015 S. Karger AG, Basel (AU)

• Processo FAPESP: 13/25787-3 - Estudo de biomarcadores de prognóstico e resposta terapêutica em tumores estromais gastrointestinais (GISTs)
• Beneficiário: Nathália Cristina Campanella
• Linha de fomento: Bolsas no Brasil - Doutorado Direto