Tissue Responses to Postoperative Laser Therapy in Diabetic Rats Submitted to Excisional Wounds

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Santana, Cristiano de Loura ^[1] ; Teixeira Silva, Daniela de Fatima ^[1] ; Deana, Alessandro Melo ^[1] ; Prates, Renato Araujo ^[1] ; Souza, Amanda Pires ^[1] ; Gomes, Mariana Teixeira ^[1] ; de Azevedo Sampaio, Brunna Pileggi ^[1] ; Shibuya, Josiane Ferraretto ^[1] ; Bussadori, Sandra Kalil ^[1] ; Mesquita-Ferrari, Raquel Agnelli ^[1] ; Santos Fernandes, Kristianne Porta ^[1] ; Franca, Cristiane Miranda ^[1]

Afiliação do(s) autor(es)

^[1] Univ Nove de Julho UNINOVE, Postgrad Program Biophoton Appl Hlth Sci, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 10, n. 4 APR 24 2015.
• Citações Web of Science: 12

Resumo

In a previous study about low-level laser therapy biomodulation on a full-thickness burn model we showed that single and fractionated dose regimens increased wound healing and leukocyte influx similarly when compared with untreated control. In order to verify if this finding would be similar in an impaired wound model, we investigated the effect of single and multiple irradiations on wound closure rate, type of inflammatory infiltrate, myofibroblasts, collagen deposition, and optical retardation of collagen in diabetic rats. Female Wistar rats in the same estrous cycle had diabetes induced with streptozotocin and an 8-mm excisional wound performed with a punch. The experimental groups were: control group - untreated ulcer; single-dose group - ulcer submitted to single dose of diode laser therapy (lambda = 660 +/- 2 nm; P = 30 mW; energy density: 4 J/cm(2)) and fractionated-dose group - ulcer submitted to 1 J/cm(2) laser therapy on Days 1, 3, 8, and 10. The ulcers were photographed on the experimental days and after euthanasia tissue samples were routinely processed for histological and immunohistochemistry analyses. Independently of the energy density, laser therapy accelerated wound closure by approximately 40% in the first three days in comparison to the control group. Laser therapy increased acute inflammatory infiltrate until Day 3. Both laser groups exhibited more myofibroblasts and better collagen organization than the control group. The findings demonstrate that low-level laser therapy in the immediate postoperative period can enhance the tissue repair process in a diabetes model. Similar effects were achieved with laser therapy applied a single time with an energy density of 4 J/cm(2) and applied four times with an energy density of 1 J/cm(2). The application of laser therapy in the inflammatory phase was the most important factor to the enhancement of the tissue repair process. (AU)

• Processo FAPESP: 12/03334-4 - Organização do colágeno no reparo cutâneo de ratos diabéticos quando tratados com laser de baixa potência: comparação entre densidades de energia
• Beneficiário: Josiane Aparecida Ferraretto Shibuya
• Linha de fomento: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 12/02801-8 - Influência de dois regimes de entrega do laser em baixa intensidade no reparo cutâneo de ratos diabéticos: avaliação morfológica, de miofibroblastos e dos colágenos I e III
• Beneficiário: Brunna Pileggi Azevedo Sampaio
• Linha de fomento: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 12/01944-0 - Estudo da ativação celular e de citocinas no reparo cutâneo de ratos diabéticos tratados com laser em baixa intensidade
• Beneficiário: Cristiane Miranda Franca
• Linha de fomento: Auxílio à Pesquisa - Regular