In vivo evaluation of the genetic toxicity of Rubus niveus Thunb. (Rosaceae) extract and initial screening of its potential chemoprevention against doxorubicin-induced DNA damage

Autor(es)

Tolentino, Flora ^[1] ; de Arai, Priscila Alves ^[1] ; Marques, Eduardo de Souza ^[1] ; Petreanu, Marcel ^[2] ; de Andrade, Sergio Faloni ^[2] ; Niero, Rivaldo ^[2] ; Perazzo, Fabio F. ^[3] ; Pires Rosa, Paulo Cesar ^[4] ; Maistro, Edson Luis ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Fac Filosofia & Ciencias, Dept Fonoaudiol, BR-17525900 Marilia, SP - Brazil

^[2] Univ Vale Itajai UNIVALI, Programa Posgrad Ciencias Farmaceut, Nucleo Invest Quim Farmaceut NIQFAR, Itajai, SC - Brazil

^[3] Univ Fed Sao Paulo UNIFESP, Inst Ciencias Ambientais Quim & Farmaceut, Dept Ciencias Exatas & Terra, Diadema, SP - Brazil

^[4] Univ Estadual Campinas UNICAMP, Fac Ciencias Med, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Ethnopharmacology; v. 164, p. 89-95, APR 22 2015.
• Citações Web of Science: 7

Resumo

Ethnopharmacological relevance: Rubus niveus Thunb. plant belongs to Rosaceae family and have been used traditionally to treat wounds, burns, inflammation, dysentery, diarrhea and for curing excessive bleeding during menstrual cycle. The present study was undertaken to investigate the in vivo genotoxicity of Rubus niveus aerial parts extract and its possible chemoprotection on doxorubicin (DXR)-induced DNA damage. In parallel, the main phytochemicals constituents in the extract were determined. Materials and methods: The animals were exposed to the extract for 24 and 48 h, and the doses selected were 500, 1000 and 2000 mg/kg b.w. administered by gavage alone or prior to DXR (30 mg/kg b.w.) administered by intraperitoneal injection. The endpoints analyzed were DNA damage in bone marrow and peripheral blood cells assessed by the alkaline alkaline (pH > 13) comet assay and bone marrow micronucleus test. Results and conclusion: The results of chemical analysis of the extract showed the presence of tormentic acid, stigmasterol, quercitinglucoronide (miquelianin) and niga-ichigoside F1 as main compounds. Both cytogenetic endpoints analyzed showed that there were no statistically significant differences (p > 0.05) between the negative control and the treated groups with the two higher doses of Rubus niveus extract alone, demonstrating absence of genotoxic and mutagenic effects. Aneugenic/clastogenic effect was observed only at 2000 mg/kg dose. On the other hand, in the both assays and all tested doses were observed a significant reduction of DNA damage and chromosomal aberrations in all groups co-treated with DXR and extract compared to those which received only DXR. These results indicate that Rubus niveus aerial parts extract did not revealed any genotoxic effect, but presented some aneugenic/clastogenic effect at higher dose; and suggest that it could be a potential adjuvant against development of second malignant neoplasms caused by the cancer chemotherapic DXR. (C) 2015 Elsevier Ireland Ltd. All rights reserved. (AU)

• Processo FAPESP: 12/17241-8 - Investigação dos efeitos citotóxico, genotóxico, antigenotóxico e antioxidante do óleo de Euterpe oleracea Mart. (açaí): análises in vitro e in vivo, histológicas, ultra-estruturais e hematológicas
• Beneficiário: Edson Luis Maistro
• Modalidade de apoio: Auxílio à Pesquisa - Regular