Genotoxicity Assessment of Reactive and Disperse Textile Dyes Using Human Dermal Equivalent (3D Cell Culture System)

Autor(es)

Leme, Daniela Morais ^{[1, 2]} ; Primo, Fernando Lucas ^[3] ; Gobo, Graciely Gomides ^[3] ; Vieira da Costa, Cleber Rafael ^[2] ; Tedesco, Antonio Claudio ^[1] ; de Oliveira, Danielle Palma ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Sao Paulo - Brazil

^[2] Univ Fed Parana UFPR, Dept Biol Celular, BR-81531980 Curitiba, Parana - Brazil

^[3] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Ctr Nanotecnol & Engn Tecidual, Dept Quim, Lab Fotobiol & Fotomed, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES; v. 78, n. 7, p. 466-480, APR 3 2015.
• Citações Web of Science: 7

Resumo

Thousands of dyes are marketed daily for different purposes, including textile dyeing. However, there are several studies reporting attributing to dyes deleterious human effects such as DNA damage. Humans may be exposed to toxic dyes through either ingestion of contaminated waters or dermal contact with colored garments. With respect to dermal exposure, human skin equivalents are promising tools to assess in vitro genotoxicity of dermally applied chemicals using a three-dimensional (3D) model to mimic tissue behavior. This study investigated the sensitivity of an in-house human dermal equivalent (DE) for detecting genotoxicity of textile dyes. Two azo (reactive green 19 {[}RG19] and disperse red 1{[}DR1]) dyes and one anthraquinone (reactive blue 2 {[}RB2]) dye were analyzed. RG19 was genotoxic for DE in a dose-responsive manner, whereas RB2 and DR1 were nongenotoxic under the conditions tested. These findings are not in agreement with previous genotoxicological assessment of these dyes carried out using two-dimensional (2D) cell cultures, which showed that DR1 was genotoxic in human hepatoma cells (HepG2) and RG19 was nongenotoxic for normal human dermal fibroblasts (NHDF). These discrepant results probably may be due to differences between metabolic activities of each cell type (organ-specific genotoxicity, HepG2 and fibroblasts) and the test setup systems used in each study (fibroblasts cultured at 2D and three-dimensional {[}3D] culture systems). Genotoxicological assessment of textile dyes in context of organ-specific genotoxicity and using in vitro models that more closely resemble in vivo tissue architecture and physiology may provide more reliable estimates of genotoxic potential of these chemicals. (AU)

• Processo FAPESP: 10/14941-3 - Avaliação de efeitos genotóxicos e mutagênicos decorrentes da exposição de corante reativo extraído de fibras de algodão por meio de suor sintético
• Beneficiário: Daniela Morais Leme
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado