Evaluation of the expression of I-FABP and L-FABP in a necrotizing enterocolitis model after the use of Lactobacillus acidophilus

Autor(es)

Lanhellas Goncalves, Frances Lilian ^[1] ; Mangueira Soares, Lucas Manoel ^[1] ; Figueira, Rebeca Lopes ^[1] ; Bertoncini Simoes, Ana Leda ^[1] ; Gallindo, Rodrigo Melo ^[1] ; Sbragia, Lourenco ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Lab Expt Fetal Surg Michael Harrison, Dept Surg & Anat, Div Pediat Surg, Ribeirao Preto Med Sch, BR-14049 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PEDIATRIC SURGERY; v. 50, n. 4, p. 543-549, APR 2015.
• Citações Web of Science: 12

Resumo

Background/purpose: Necrotizing enterocolitis (NEC) is a severe intestinal disease that primarily affects premature babies, leading to high mortality and morbidity. Probiotics represent an important alternative prophylaxis for NEC but its mechanism of action is poorly understood. Moreover, intestinal and liver-type fatty acid-binding proteins (I-FABP and L-FABP) may be utilized because markers of intestinal injury, including NEC. We aimed to evaluate the protection induced by the Lactobacillus acidophilus on the intestines of newborn rats submitted to experimental NEC using I-FABP and L-FABP as biomarkers. Methods: Sprague-Dawley newborn rats were divided into three groups: (1) C (control)-breast-fed; (2) NEC-subjected to NEC protocol and (3) NECP-NEC + probiotic. Morphometric, intestinal lesion, immunohistochemistry and Western blotting analysis were performed. Statistical significant differences were considered when p < 0.05. Results: Intestinal weight was lower in NEC and NECP compared to C (p < 0.05). Intestinal injury was lower in NECP compared to NEC. Prophylactic probiotic recovered mucosa and muscular layers' thickness to C levels (p < 0.05). I-FABP and L-FABP expressions in NECP showed intermediate values between C and NEC. Conclusion: L. acidophilus had a protective effect on the development of NEC and FABPs could demonstrate the degree of tissular damage of the intestine. (C) 2015 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 11/00794-1 - Avaliação da via do óxido nítrico in vivo após a oclusão traqueal fetal sobre a expressão de receptores de VEGF pulmonar na hérnia diafragmática congênita em ratos induzida pelo Nitrofen
• Beneficiário: Lourenço Sbragia Neto
• Modalidade de apoio: Auxílio à Pesquisa - Regular