beta-Lactamase-based biosensor for the electrochemical determination of benzylpenicillin in milk

Autor(es)

do Prado, Thiago M. ^[1] ; Foguel, Marcos V. ^[1] ; Goncalves, Luis Moreira ^[2] ; Sotomayor, Maria del Pilar T. ^[1]

Afiliação do(s) autor(es)

^[1] IQ UNESP, Araraquara, SP - Brazil

^[2] Univ Porto, Fac Ciencias, Dept Quim & Bioquim, P-4169007 Oporto - Portugal

• Tipo de documento: Artigo Científico
• Fonte: SENSORS AND ACTUATORS B-CHEMICAL; v. 210, p. 254-258, APR 2015.
• Citações Web of Science: 12

Resumo

Antibiotics are widely used in dairy cattle production, however this can pose a public health threat, particularly when antibiotic residues remain in the food products like milk. Therefore, new analytical solutions aiming the determination of antibiotics, like the still commonly used penicillin, are welcomed. This work describes a novel electrochemical biosensor, with amperometric and differential pulse voltammetric detection, based on a modified carbon paste with beta-lactamase for the determination of penicillin G, a beta-lactam antibiotic. It is a `second-generation' biosensor since cobalt phthalocyanine was used as an electron mediator. The proposed biosensor, by differential pulse voltammetry, presented a limit of detection of 0.079 mu mol/L. Using this biosensor, a value of 43 +/- 3 mu mol/L was determined for the Michaelis-Menten enzymatic constant for penicillinase extracted from Bacillus cereus. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 11/17552-0 - Detecção e quantificação de corantes de importância ambiental empregando sensores biomiméticos baseados em fibras ópticas e polímeros molecularmente impressos
• Beneficiário: Marcos Vinicius Foguel
• Linha de fomento: Bolsas no Brasil - Doutorado