Genetic and non-genetic factors that increase the risk of non-syndromic cleft lip and/or palate development

Autor(es):Mostrar menos -

Bezerra, J. F. ^[1] ; Oliveira, G. H. M. ^[1] ; Soares, C. D. ^[1] ; Cardoso, M. L. ^[1] ; Ururahy, M. A. G. ^[1] ; Neto, F. P. F. ^[1] ; Lima-Neto, L. G. ^[2] ; Luchessi, A. D. ^[1] ; Silbiger, V. N. ^[1] ; Fajardo, C. M. ^[3] ; de Oliveira, S. R. ^[4] ; Almeida, M. das G. ^[1] ; Hirata, R. D. C. ^[3] ; de Rezende, A. A. ^[1] ; Hirata, M. H. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Fed Rio Grande do Norte, Dept Clin & Toxicol Anal, BR-59072970 Natal, RN - Brazil

^[2] Univ CEUMA, Sao Luis, MA - Brazil

^[3] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, Sao Paulo, SP - Brazil

^[4] Univ Fed Rio Grande do Norte, Pediat Hosp Prof Heriberto Ferreira Bezerra, Program Children Cleft Lip & Palate, BR-59072970 Natal, RN - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ORAL DISEASES; v. 21, n. 3, p. 393-399, APR 2015.
• Citações Web of Science: 20

Resumo

ObjectivesWe investigated the relationship between non-syndromic cleft lip/palate (NSCLP) and polymorphisms in methylenetetrahydrofolate reductase (MTHFR), methionine synthase (MTR), methionine synthase reductase (MTRR), and RFC1, as well as the corresponding interactions with environmental factors. Subjects and MethodsOne hundred and forty NSCLP patients and their mothers, as well as 175 control individuals and their mothers, were recruited. Information regarding smoking and alcohol consumption was recorded. Blood samples were obtained in order to measure serum folate and cobalamin, as well as, plasma total homocysteine concentrations and to extract DNA. Polymorphisms in MTHFR(677C>T and 1298A>C), MTR(2756A>G), MTR(66A>G), and RFC1(80A>G) were analyzed by PCR-restriction fragment length polymorphism. ResultsAmong the patients, 59.5% had cleft lip and palate, 22.0% had cleft palate, and 18.5% had cleft lip only. Maternal alcohol consumption and reduced folic acid concentrations in both children and mothers (P<0.001 and P=0.003, respectively) were risk factors for NSCLP. Patients and their mothers carrying the MTHFR 667T allele showed lower serum folate than CC (P=0.011 and P=0.030, respectively). Mothers who carried the MTHFR 1298C allele exhibited increased risk of having a child with NSCLP, after adjusting for alcohol consumption (OR: 1.75, 95% CI: 1.03-2.99, P=0.038). ConclusionsReduced folic acid levels, alcohol consumption, and the MTHFR 677T and 1298C alleles may have contributed to NSCLP development in this sample population from Rio Grande do Norte. (AU)

• Processo FAPESP: 08/05064-9 - Avaliação de polimorfismos genéticos associados ao metabolismo do ácido fólico e ao desenvolvimento facial em crianças portadoras de fendas orais no estado do RN
• Beneficiário: Mario Hiroyuki Hirata
• Linha de fomento: Auxílio à Pesquisa - Regular