Improvement on sugar cane bagasse hydrolysis using enzymatic mixture designed cocktail

Autor(es)

Bussamra, Bianca Consorti ^[1] ; Freitas, Sindelia ^[1] ; da Costa, Aline Carvalho ^[2]

Afiliação do(s) autor(es)

^[1] Brazilian Ctr Res Energy & Mat, Brazilian Bioethanol Sci & Technol Lab CTBE, BR-13083970 Campinas, SP - Brazil

^[2] Univ Campinas UNICAMP, Sch Chem Engn, BR-13083852 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Bioresource Technology; v. 187, p. 173-181, JUL 2015.
• Citações Web of Science: 16

Resumo

The aim of this work was to study cocktail supplementation for sugar cane bagasse hydrolysis, where the enzymes were provided from both commercial source and microorganism cultivation (Trichoderma reesei and genetically modified Escherichia coli), followed by purification. Experimental simplex lattice mixture design was performed to optimize the enzymatic proportion. The response was evaluated through hydrolysis microassays validated here. The optimized enzyme mixture, comprised of T. reesei fraction (80%), endoglucanase (10%) and beta-glucosidase (10%), converted, theoretically, 72% of cellulose present in hydrothermally pretreated bagasse, whereas commercial Celluclast 1.5 L converts 49.11% +/- 0.49. Thus, a rational enzyme mixture designed by using synergism concept and statistical analysis was capable of improving biomass saccharification. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 10/08089-2 - Desenvolvimento de uma plataforma de expressão, baseada em E. coli, para a produção de enzimas envolvidas na hidrólise de biomassas lignocelulósicas
• Beneficiário: Sindelia Freitas Azzoni
• Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
• Processo FAPESP: 08/57873-8 - Um processo integrado para produção total de bioetanol e emissão zero de CO2
• Beneficiário: Rubens Maciel Filho
• Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 12/23223-2 - Desenvolvimento de coquetéis enzimáticos otimizados para hidrólise de bagaço de cana-de-açúcar
• Beneficiário: Bianca Consorti Bussamra
• Linha de fomento: Bolsas no Brasil - Mestrado