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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Improvement on sugar cane bagasse hydrolysis using enzymatic mixture designed cocktail

Full text
Author(s):
Bussamra, Bianca Consorti [1] ; Freitas, Sindelia [1] ; da Costa, Aline Carvalho [2]
Total Authors: 3
Affiliation:
[1] Brazilian Ctr Res Energy & Mat, Brazilian Bioethanol Sci & Technol Lab CTBE, BR-13083970 Campinas, SP - Brazil
[2] Univ Campinas UNICAMP, Sch Chem Engn, BR-13083852 Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Bioresource Technology; v. 187, p. 173-181, JUL 2015.
Web of Science Citations: 16
Abstract

The aim of this work was to study cocktail supplementation for sugar cane bagasse hydrolysis, where the enzymes were provided from both commercial source and microorganism cultivation (Trichoderma reesei and genetically modified Escherichia coli), followed by purification. Experimental simplex lattice mixture design was performed to optimize the enzymatic proportion. The response was evaluated through hydrolysis microassays validated here. The optimized enzyme mixture, comprised of T. reesei fraction (80%), endoglucanase (10%) and beta-glucosidase (10%), converted, theoretically, 72% of cellulose present in hydrothermally pretreated bagasse, whereas commercial Celluclast 1.5 L converts 49.11% +/- 0.49. Thus, a rational enzyme mixture designed by using synergism concept and statistical analysis was capable of improving biomass saccharification. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 08/57873-8 - An integrated process for total bioethanol production and zero CO2 emission
Grantee:Rubens Maciel Filho
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
FAPESP's process: 10/08089-2 - Development of an expression platform, based on E. coli, for the production of enzymes used for lignocellulosic biomass hydrolysis
Grantee:Sindelia Freitas Azzoni
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 12/23223-2 - Development of optimized enzyme cocktails for hydrolysis of sugar cane bagasse
Grantee:Bianca Consorti Bussamra
Support Opportunities: Scholarships in Brazil - Master