Enhanced detection of viral diversity using partial and near full-length genomes of human immunodeficiency virus Type 1 provirus deep sequencing data from recently infected donors at four blood centers in Brazil

Autor(es):Mostrar menos -

Pessoa, Rodrigo ^[1] ; Watanabe, Jaqueline Tomoko ^[1] ; Calabria, Paula ^[1] ; Alencar, Cecilia Salete ^[2] ; Loureiro, Paula ^[3] ; Lopes, Maria Esther ^[4] ; Proetti, Anna Barbara ^[5] ; Felix, Alvina Clara ^[1] ; Sabino, Ester C. ^[6] ; Busch, Michael P. ^[7] ; Sanabani, Sabri S. ^[2] ; Recipient, Int Component NHLBI

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sao Paulo Inst Trop Med, Dept Virol, BR-05403000 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Sch Med, Dept Pathol, Clin Lab, LIM 03, HC, BR-05403000 Sao Paulo, SP - Brazil

^[3] Pernambuco State Ctr Hematol & Hemotherapy HEMOPE, Recife, PE - Brazil

^[4] Hemorio, Rio De Janeiro - Brazil

^[5] Minas Gerais State Ctr Hematol & Hemotherapy HEMO, Belo Horizonte, MG - Brazil

^[6] Univ Sao Paulo, Inst Trop Med, Dept Infect Dis, BR-05403000 Sao Paulo, SP - Brazil

^[7] Blood Syst Res Inst, San Francisco, CA - USA

• Tipo de documento: Artigo Científico
• Fonte: Transfusion; v. 55, n. 5, p. 980-990, MAY 2015.
• Citações Web of Science: 7

Resumo

BackgroundHere, we report application of high-throughput near full-length genome (NFLG) and partial human immunodeficiency virus Type 1 (HIV-1) proviral genome deep sequencing to characterize HIV in recently infected blood donors at four major blood centers in Brazil. Study Design and MethodsFrom 2007 to 2011, a total of 341 HIV+ blood donors from four blood centers were recruited to participate in a case-control study to identify HIV risk factors and motivations to donate. Forty-seven (17 from SAo Paulo, eight from Minas Gerais, 11 from Pernambuco, and 11 from Rio de Janeiro) were classified as recently infected based on testing by less-sensitive enzyme immunoassays. Five overlapping amplicons spanning the HIV genome were polymerase chain reaction amplified from peripheral blood mononuclear cells. The amplicons were molecularly barcoded, pooled, and sequenced by a paired-end protocol (Illumina). ResultsOf the 47 recently infected donor samples studied, 39 (82.9%) NFLGs and six (12.7%) partial fragments were de novo assembled into contiguous sequences and successfully subtyped. Subtype B was the only nonrecombinant virus identified in this study and accounted for 62.2% (28/45) of samples. The remaining 37.8% (17/45) of samples showed various patterns of subtype discordance in different regions of HIV-1 genomes, indicating two to four circulating recombinant subtypes derived from Clades B, F, and C. Fourteen samples (31.1%) from this study harbored drug resistance mutations, indicating higher rate of drug resistance among Brazilian blood donors. ConclusionOur findings revealed a high proportion of HIV-1 recombinants among recently infected blood donors in Brazil, which has implications for future blood screening, diagnosis, therapy, and vaccine development. (AU)

• Processo FAPESP: 11/11090-5 - Caracterização do vírus da imunodeficiência humana (HIV-1) pela análise do genoma completo viral em amostras de doadores de sangue nos estados de Pernambuco, Minas Gerais, Rio de Janeiro e São Paulo
• Beneficiário: Sabri Saeed Mohammed Ahmed Al-Sanabani
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/12297-2 - Análise dos transcritos de miRNAs, REX e tax em células T no curso da infecção pelo HTLV-1, utilizando sequenciamento de nova geração
• Beneficiário: Sabri Saeed Mohammed Ahmed Al-Sanabani
• Modalidade de apoio: Auxílio à Pesquisa - Regular