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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Enhanced detection of viral diversity using partial and near full-length genomes of human immunodeficiency virus Type 1 provirus deep sequencing data from recently infected donors at four blood centers in Brazil

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Pessoa, Rodrigo [1] ; Watanabe, Jaqueline Tomoko [1] ; Calabria, Paula [1] ; Alencar, Cecilia Salete [2] ; Loureiro, Paula [3] ; Lopes, Maria Esther [4] ; Proetti, Anna Barbara [5] ; Felix, Alvina Clara [1] ; Sabino, Ester C. [6] ; Busch, Michael P. [7] ; Sanabani, Sabri S. [2] ; Recipient, Int Component NHLBI
Total Authors: 12
Affiliation:
[1] Univ Sao Paulo, Sao Paulo Inst Trop Med, Dept Virol, BR-05403000 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Sch Med, Dept Pathol, Clin Lab, LIM 03, HC, BR-05403000 Sao Paulo, SP - Brazil
[3] Pernambuco State Ctr Hematol & Hemotherapy HEMOPE, Recife, PE - Brazil
[4] Hemorio, Rio De Janeiro - Brazil
[5] Minas Gerais State Ctr Hematol & Hemotherapy HEMO, Belo Horizonte, MG - Brazil
[6] Univ Sao Paulo, Inst Trop Med, Dept Infect Dis, BR-05403000 Sao Paulo, SP - Brazil
[7] Blood Syst Res Inst, San Francisco, CA - USA
Total Affiliations: 7
Document type: Journal article
Source: Transfusion; v. 55, n. 5, p. 980-990, MAY 2015.
Web of Science Citations: 7
Abstract

BackgroundHere, we report application of high-throughput near full-length genome (NFLG) and partial human immunodeficiency virus Type 1 (HIV-1) proviral genome deep sequencing to characterize HIV in recently infected blood donors at four major blood centers in Brazil. Study Design and MethodsFrom 2007 to 2011, a total of 341 HIV+ blood donors from four blood centers were recruited to participate in a case-control study to identify HIV risk factors and motivations to donate. Forty-seven (17 from SAo Paulo, eight from Minas Gerais, 11 from Pernambuco, and 11 from Rio de Janeiro) were classified as recently infected based on testing by less-sensitive enzyme immunoassays. Five overlapping amplicons spanning the HIV genome were polymerase chain reaction amplified from peripheral blood mononuclear cells. The amplicons were molecularly barcoded, pooled, and sequenced by a paired-end protocol (Illumina). ResultsOf the 47 recently infected donor samples studied, 39 (82.9%) NFLGs and six (12.7%) partial fragments were de novo assembled into contiguous sequences and successfully subtyped. Subtype B was the only nonrecombinant virus identified in this study and accounted for 62.2% (28/45) of samples. The remaining 37.8% (17/45) of samples showed various patterns of subtype discordance in different regions of HIV-1 genomes, indicating two to four circulating recombinant subtypes derived from Clades B, F, and C. Fourteen samples (31.1%) from this study harbored drug resistance mutations, indicating higher rate of drug resistance among Brazilian blood donors. ConclusionOur findings revealed a high proportion of HIV-1 recombinants among recently infected blood donors in Brazil, which has implications for future blood screening, diagnosis, therapy, and vaccine development. (AU)

FAPESP's process: 11/11090-5 - Complete genomes for HIV: viral genetic diversity among seropositive first-time blood donors in four blood centres in Brazil
Grantee:Sabri Saeed Mohammed Ahmed Al-Sanabani
Support Opportunities: Regular Research Grants
FAPESP's process: 11/12297-2 - Profiling the human T-cells miRNA, REX and tax transcriptomes in the course of HTLV-1 infection using a deep sequencing approach
Grantee:Sabri Saeed Mohammed Ahmed Al-Sanabani
Support Opportunities: Regular Research Grants