Revealing praziquantel molecular targets using mass spectrometry imaging: an expeditious approach applied to Schistosoma mansoni

Autor(es)

Ferreira, Monica Siqueira ^[1] ; de Oliveira, Rosimeire Nunes ^[2] ; de Oliveira, Diogo Noin ^[1] ; Esteves, Cibele Zanardi ^[1] ; Allegretti, Silmara Marques ^[2] ; Catharino, Rodrigo Ramos ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Innovare Biomarkers Lab, Med & Expt Surg Nucleus, Sao Paulo - Brazil

^[2] Univ Estadual Campinas, Dept Anim Biol, Inst Biol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Journal for Parasitology; v. 45, n. 6, p. 385-391, MAY 2015.
• Citações Web of Science: 10

Resumo

Finding specific molecular targets and the mechanism of action of praziquantel in the treatment of schistosomiasis remains a challenging task. Our efforts were focused on obtaining further information on worm composition before and after exposure to praziquantel in the treatment of schistosomiasis to elucidate the potential sites of action of this drug. Evidence indicates that the lipid bilayer is changed by treatment with praziquantel. Following this rationale, we employed a mass spectrometry imaging-based approach that helped to characterise lipids in specific locations, which are directly involved in the biochemical pathways of the BH strain of Schistosoma mansoni, as well as differentiating the molecular response that each worm sex presents in vivo. Our findings demonstrated significant differences between the chemical markers found in adult worms before and after praziquantel exposure, especially in phospholipids, which were predominantly identified as chemical markers in all samples. Results also indicate that distinct molecular pathways in both male and female worms could be differentially affected by praziquantel treatment. These data shine new light on the mechanism of action of praziquantel, taking a further step towards its full understanding. (C) 2015 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 14/00302-0 - Shotgun lipidômico em giardíase, meningites e tuberculose: a busca de marcadores para diagnósticos, empregando novas e revolucionárias técnicas de espectrometria de massas
• Beneficiário: Cibele Zanardi Esteves
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/50400-0 - Metabolismo energético, estado redox e funcionalidade mitocondrial na morte celular e em desordens cardiometabólicas e neurodegenerativas
• Beneficiário: Aníbal Eugênio Vercesi
• Modalidade de apoio: Auxílio à Pesquisa - Temático