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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Revealing praziquantel molecular targets using mass spectrometry imaging: an expeditious approach applied to Schistosoma mansoni

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Autor(es):
Ferreira, Monica Siqueira [1] ; de Oliveira, Rosimeire Nunes [2] ; de Oliveira, Diogo Noin [1] ; Esteves, Cibele Zanardi [1] ; Allegretti, Silmara Marques [2] ; Catharino, Rodrigo Ramos [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Innovare Biomarkers Lab, Med & Expt Surg Nucleus, Sao Paulo - Brazil
[2] Univ Estadual Campinas, Dept Anim Biol, Inst Biol, Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: International Journal for Parasitology; v. 45, n. 6, p. 385-391, MAY 2015.
Citações Web of Science: 10
Resumo

Finding specific molecular targets and the mechanism of action of praziquantel in the treatment of schistosomiasis remains a challenging task. Our efforts were focused on obtaining further information on worm composition before and after exposure to praziquantel in the treatment of schistosomiasis to elucidate the potential sites of action of this drug. Evidence indicates that the lipid bilayer is changed by treatment with praziquantel. Following this rationale, we employed a mass spectrometry imaging-based approach that helped to characterise lipids in specific locations, which are directly involved in the biochemical pathways of the BH strain of Schistosoma mansoni, as well as differentiating the molecular response that each worm sex presents in vivo. Our findings demonstrated significant differences between the chemical markers found in adult worms before and after praziquantel exposure, especially in phospholipids, which were predominantly identified as chemical markers in all samples. Results also indicate that distinct molecular pathways in both male and female worms could be differentially affected by praziquantel treatment. These data shine new light on the mechanism of action of praziquantel, taking a further step towards its full understanding. (C) 2015 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 14/00302-0 - Shotgun lipidômico em giardíase, meningites e tuberculose: a busca de marcadores para diagnósticos, empregando novas e revolucionárias técnicas de espectrometria de massas
Beneficiário:Cibele Zanardi Esteves
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 11/50400-0 - Metabolismo energético, estado redox e funcionalidade mitocondrial na morte celular e em desordens cardiometabólicas e neurodegenerativas
Beneficiário:Aníbal Eugênio Vercesi
Linha de fomento: Auxílio à Pesquisa - Temático