Optimization of Amine-Rich Multilayer Thin Films for the Capture and Quantification of Prostate-Specific Antigen

Autor(es)

Polak, Roberta ^{[1, 2]} ; Bradwell, Grinia M. ^[1] ; Gilbert, Jonathan B. ^[3] ; Danielsen, Scott ^[4] ; Beppu, Marisa M. ^[5] ; Cohen, Robert E. ^[3] ; Rubner, Michael F. ^[1]

Afiliação do(s) autor(es)

^[1] MIT, Dept Mat Sci & Engn, Cambridge, MA 02139 - USA

^[2] Univ Sao Paulo, Sch Pharmaceut Sci, Sao Paulo, SP - Brazil

^[3] MIT, Dept Chem Engn, Cambridge, MA 02139 - USA

^[4] Univ Penn, Dept Chem & Biomol Engn, Philadelphia, PA 19104 - USA

^[5] Univ Campinas UNICAMP, Sch Chem Engn, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Langmuir; v. 31, n. 19, p. 5479-5488, MAY 19 2015.
• Citações Web of Science: 6

Resumo

It is demonstrated that poly(allylamine hydrochloride)/poly(styrenesulfonate) (PAH/SPS) multilayer films can be successfully tailored for the capture and detection of small biomolecules in dilute concentrations. Based on in vitro results, these films could be potentially applied for rapid and high-throughput diagnosis of dilute biomarkers in serum or tissue. PAH presents functional amino groups that can be further reacted with desired chemistries in order to create customizable and specific surfaces for biomolecule capture. A variety of film assembly characteristics were tested (pH, molecular weight of PAH, and ionic strength) to tune the biotinylation and swelling behavior of these films to maximize detection capabilities. The resultant optimized biotinylated PAH/SPS 9.3/9.3 system was utilized in conjunction with quantum dots (Qdots) to capture and detect a dilute biomarker for prostate cancer, prostate-specific antigen (PSA). Compared to previous work, our system presents a good sensitivity for PSA detection within the clinically relevant range of 0.4-100 ng/mL. (AU)

• Processo FAPESP: 10/51066-3 - Desenvolvimento e fabricacao de filmes ultra-finos, obtidos pela tecnica layer-by-layer, para aplicacoes na entrega direcionada de farmacos e na captura seletiva de bio-marcadores.
• Beneficiário: Roberta Polak
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/21326-6 - Desenvolvimento de filme funcional via técnica de layer-by-layer para liberação de droga antineoplásica encapsulada em lipossomas ecogênicos
• Beneficiário: Roberta Polak
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado