| Full text | |
| Author(s): |
Polak, Roberta
[1, 2]
;
Bradwell, Grinia M.
[1]
;
Gilbert, Jonathan B.
[3]
;
Danielsen, Scott
[4]
;
Beppu, Marisa M.
[5]
;
Cohen, Robert E.
[3]
;
Rubner, Michael F.
[1]
Total Authors: 7
|
| Affiliation: | [1] MIT, Dept Mat Sci & Engn, Cambridge, MA 02139 - USA
[2] Univ Sao Paulo, Sch Pharmaceut Sci, Sao Paulo, SP - Brazil
[3] MIT, Dept Chem Engn, Cambridge, MA 02139 - USA
[4] Univ Penn, Dept Chem & Biomol Engn, Philadelphia, PA 19104 - USA
[5] Univ Campinas UNICAMP, Sch Chem Engn, Campinas, SP - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | Langmuir; v. 31, n. 19, p. 5479-5488, MAY 19 2015. |
| Web of Science Citations: | 6 |
| Abstract | |
It is demonstrated that poly(allylamine hydrochloride)/poly(styrenesulfonate) (PAH/SPS) multilayer films can be successfully tailored for the capture and detection of small biomolecules in dilute concentrations. Based on in vitro results, these films could be potentially applied for rapid and high-throughput diagnosis of dilute biomarkers in serum or tissue. PAH presents functional amino groups that can be further reacted with desired chemistries in order to create customizable and specific surfaces for biomolecule capture. A variety of film assembly characteristics were tested (pH, molecular weight of PAH, and ionic strength) to tune the biotinylation and swelling behavior of these films to maximize detection capabilities. The resultant optimized biotinylated PAH/SPS 9.3/9.3 system was utilized in conjunction with quantum dots (Qdots) to capture and detect a dilute biomarker for prostate cancer, prostate-specific antigen (PSA). Compared to previous work, our system presents a good sensitivity for PSA detection within the clinically relevant range of 0.4-100 ng/mL. (AU) | |
| FAPESP's process: | 11/21326-6 - Development of functional patches via layer-by-layer for the release of anticancer drug encapsulated in echogenic liposomes |
| Grantee: | Roberta Polak |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate |