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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

In Vitro and in Vivo Studies of Novel Poly(D,L-lactic acid), Superhydrophilic Carbon Nanotubes, and Nanohydroxyapatite Scaffolds for Bone Regeneration

Texto completo
Autor(es):
Siqueira, Idalia A. W. B. [1] ; Corat, Marcus Alexandre F. [2] ; Cavalcanti, Bruno das Neves [3] ; Ribeiro Neto, Wilson Alves [4] ; Martin, Airton Abrahao [5] ; Suman Bretas, Rosario Elida [4] ; Marciano, Fernanda Roberta [1] ; Lobo, Anderson Oliveira [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Vale Paraiba UNIVAP, Inst Res & Dev IP&D 2, Lab Biomed Nanotechnol NANOBIO, BR-12244000 Sao Paulo - Brazil
[2] Univ Estadual Campinas, Multidisciplinary Ctr Biol Invest, Lab Anim Sci CEMLB, BR-13083970 Sao Paulo - Brazil
[3] Univ Michigan, Dept Cariol Restorat Sci & Endodont, Sch Dent, Ann Arbor, MI 48109 - USA
[4] Univ Fed Sao Carlos, Dept Mat Engn, BR-13565905 Sao Paulo - Brazil
[5] Univ Vale Paraiba UNIVAP, Inst Res & Dev IP&D 2, Lab Biomed Vibrat Spect, BR-12244000 Sao Paulo - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: ACS APPLIED MATERIALS & INTERFACES; v. 7, n. 18, p. 9385-9398, MAY 13 2015.
Citações Web of Science: 29
Resumo

Poly(D,L-lactide acid, PDLLA) has been researched for scaffolds in bone regeneration. However, its hydrophobocity and smooth surface impedes its interaction with biological fluid and cell adhesion. To alter the surface characteristics, different surface Modification techniques have been developed to facilitate biological application. The present study compared two different routes to produce PDLLA/superhydrophilic vertically aligned carbon nanotubes:nanohydroxyapatite (PDLLA/VACNT-O:nHAp) scaffolds. For this, we used electrodeposition and immersion in simulated body fluid (SBF). Characterization by goniometry, scanning electron microscopy, X-ray diffraction, and infrared spectroscopy confirmed the polymer modifications, the in vitro bioactivity, and biomineralization. Differential sunning calorimetry and thermal gravimetric analyses showed that the inclusion of VACNT-O:nHA probably acts as a nucleating agent increasing the crystallization rate in the neat PDLLA without structural alteration. Our results showed the formation of a dense nHAp layer on all scaffolds after 14 days of immersion in SBF solution; the most intense carbonated nHAp peaks observed in the PDLLA/VACNT-O:nHAp samples suggest higher calcium precipitation compared to the PDLLA control. Both cell viability and alkaline phosphatase assays showed favorable results, because no cytotoxic effects were present and all produced scaffolds were able to induce detectable mineralization. Bone defects were used to evaluate the bone regeneration; the confocal Raman and histological results confirmed high potential for bone applications: In vivo study showed that the PDLLA/VACNT-O:nHAp scaffolds mimicked the immature bone and induced bone remodeling: These findings indicate surface improvement and the applicability of this new nanobiomaterial for hone regenerative medicine. (AU)

Processo FAPESP: 12/02159-4 - Análise da biomineralização e proliferação celular in vitro de scaffolds de nanohidroxyapatita/nanotubos de carbono verticalmente alinhados
Beneficiário:Tayra Rodrigues Brazil
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 11/20345-7 - Estudo de filmes de carbono-tipo diamante contendo nanopartículas incorporadas para aplicações biomédicas
Beneficiário:Fernanda Roberta Marciano
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
Processo FAPESP: 11/17877-7 - Desenvolvimento de novos scaffolds poliméricos por eletrofiação com incorporação de nanotubos alinhados e nanohidroxiapatita para regeneração óssea
Beneficiário:Anderson de Oliveira Lobo
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores