Responses of Dental Pulp Cells to a Less Invasive Bleaching Technique Applied to Adhesively Restored Teeth

Autor(es)

Soares, Diana Gabriela ^[1] ; Sacono, Nancy Tomoko ^[2] ; Ribeiro, Ana Paula Dias ^{[3, 4]} ; Basso, Fernanda Goncalves ^[1] ; Scheffel, Debora Sales ^[5] ; Hebling, Josimeri ^[5] ; de Souza Costa, Carlos Alberto ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, Araraquara Sch Dent, Dept Physiol & Pathol, Sao Paulo - Brazil

^[2] Univ Fed Goias, Dept Stomatol & Oral Pathol, Goias, Go - Brazil

^[3] Fed Univ Brasilia, Dept Dent, Brasilia, DF - Brazil

^[4] Campus Univ Darcy Ribeiro, Brasilia, DF - Brazil

^[5] Univ Estadual Paulista, UNESP, Araraquara Sch Dent, Dept Orthodont & Pediat Dent, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Adhesive Dentistry; v. 17, n. 2, p. 155-161, 2015.
• Citações Web of Science: 3

Resumo

Purpose: To assess the cytotoxicity of 35% hydrogen peroxide (HP) bleaching gel applied for 15 min to sound or restored teeth with two-step self-etching adhesive systems and composite resin. Materials and Methods: Sound and restored enamel/dentin disks were stored in water for 24 h or 6 months + thermocycling. The disks were adapted to artificial pulp chambers and placed in compartments containing culture medium. Immediately after bleaching, the culture medium in contact with dentin was applied for 1 h to previously cultured odontoblast-like MDPC-23 cells. Thereafter, cell viability (MTT assay) and morphology (SEM) were assessed. Data were analyzed by two-way ANOVA and Tukey's test (alpha = 5%). Results: In comparison to the negative control group (no treatment), no significant cell viability reduction occurred in those groups in which sound teeth were bleached. However, a significant decrease in cell viability was observed in the adhesive-restored bleached groups compared to negative control. No significant difference among bleached groups was observed with respect to the presence of restoration and storage time. Conclusion: The application of 35% HP bleaching gel to sound teeth for 15 min does not cause toxic effects in pulp cells. When this bleaching protocol was performed in adhesive-restored teeth, a significant toxic effect occurred. (AU)

• Processo FAPESP: 13/23520-0 - Bioatividade de scaffolds experimentais a base de quitosana e colágeno sobre cultura de células pulpares humanas
• Beneficiário: Diana Gabriela Soares dos Passos
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado