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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Responses of Dental Pulp Cells to a Less Invasive Bleaching Technique Applied to Adhesively Restored Teeth

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Author(s):
Soares, Diana Gabriela [1] ; Sacono, Nancy Tomoko [2] ; Ribeiro, Ana Paula Dias [3, 4] ; Basso, Fernanda Goncalves [1] ; Scheffel, Debora Sales [5] ; Hebling, Josimeri [5] ; de Souza Costa, Carlos Alberto [1]
Total Authors: 7
Affiliation:
[1] Univ Estadual Paulista, UNESP, Araraquara Sch Dent, Dept Physiol & Pathol, Sao Paulo - Brazil
[2] Univ Fed Goias, Dept Stomatol & Oral Pathol, Goias, Go - Brazil
[3] Fed Univ Brasilia, Dept Dent, Brasilia, DF - Brazil
[4] Campus Univ Darcy Ribeiro, Brasilia, DF - Brazil
[5] Univ Estadual Paulista, UNESP, Araraquara Sch Dent, Dept Orthodont & Pediat Dent, Sao Paulo - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Journal of Adhesive Dentistry; v. 17, n. 2, p. 155-161, 2015.
Web of Science Citations: 3
Abstract

Purpose: To assess the cytotoxicity of 35% hydrogen peroxide (HP) bleaching gel applied for 15 min to sound or restored teeth with two-step self-etching adhesive systems and composite resin. Materials and Methods: Sound and restored enamel/dentin disks were stored in water for 24 h or 6 months + thermocycling. The disks were adapted to artificial pulp chambers and placed in compartments containing culture medium. Immediately after bleaching, the culture medium in contact with dentin was applied for 1 h to previously cultured odontoblast-like MDPC-23 cells. Thereafter, cell viability (MTT assay) and morphology (SEM) were assessed. Data were analyzed by two-way ANOVA and Tukey's test (alpha = 5%). Results: In comparison to the negative control group (no treatment), no significant cell viability reduction occurred in those groups in which sound teeth were bleached. However, a significant decrease in cell viability was observed in the adhesive-restored bleached groups compared to negative control. No significant difference among bleached groups was observed with respect to the presence of restoration and storage time. Conclusion: The application of 35% HP bleaching gel to sound teeth for 15 min does not cause toxic effects in pulp cells. When this bleaching protocol was performed in adhesive-restored teeth, a significant toxic effect occurred. (AU)

FAPESP's process: 13/23520-0 - Bioactivity of experimental scaffolds of chitosan and collagen on cultured human dental pulp cells
Grantee:Diana Gabriela Soares dos Passos
Support Opportunities: Scholarships in Brazil - Post-Doctoral