Circulating endothelial cells are increased in chronic myeloid leukemia blast crisis

Autor(es)

Godoy, C. R. T. ^[1] ; Levy, D. ^[2] ; Giampaoli, V. ^[3] ; Chamone, D. A. F. ^[1] ; Bydlowski, S. P. ^[2] ; Pereira, J. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Lab Genet & Hematol Mol, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Inst Matemat & Estat, Dept Estat, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Medical and Biological Research; v. 48, n. 6, p. 509-514, JUN 2015.
• Citações Web of Science: 5

Resumo

We measured circulating endothelial precursor cells (EPCs), activated circulating endothelial cells (aCECs), and mature circulating endothelial cells (mCECs) using four-color multiparametric flow cytometry in the peripheral blood of 84 chronic myeloid leukemia (CML) patients and 65 healthy controls; and vascular endothelial growth factor (VEGF) by quantitative real-time PCR in 50 CML patients and 32 healthy controls. Because of an increase in mCECs, the median percentage of CECs in CML blast crisis (0.0146%) was significantly higher than in healthy subjects (0.0059%, P<0.01) and in the accelerated phase (0.0059%, P=0.01). There were no significant differences in the percentages of CECs in chronic- or active-phase patients and healthy subjects (P>0.05). In addition, VEGF gene expression was significantly higher in all phases of CML: 0.245 in blast crisis, 0.320 in the active phase, and 0.330 in chronic phase patients than it was in healthy subjects (0.145). In conclusion, CML in blast crisis had increased levels of CECs and VEGF gene expression, which may serve as markers of disease progression and may become targets for the management of CML. (AU)

• Processo FAPESP: 06/55572-5 - Quantificacao de celulas endoteliais em portadores de leucemia mieloide cronica por citometria de fluxo.
• Beneficiário: Juliana Pereira
• Modalidade de apoio: Auxílio à Pesquisa - Regular