In vitro effects of thiamethoxam on larvae of Africanized honey bee Apis mellifera (Hymenoptera: Apidae)

Autor(es)

Tavares, Daiana Antonia ^[1] ; Roat, Thaisa Cristina ^[1] ; Carvalho, Stephan Malfitano ^[2] ; Mathias Silva-Zacarin, Elaine Cristina ^[3] ; Malaspina, Osmar ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Dept Biol, BR-13506900 Rio Claro, SP - Brazil

^[2] Univ Fed Uberlandia, Inst Ciencias Agr, BR-38400 Uberlandia, MG - Brazil

^[3] Univ Fed Sao Carlos, Dept Biol, Sorocaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Chemosphere; v. 135, p. 370-378, SEP 2015.
• Citações Web of Science: 27

Resumo

Several investigations have revealed the toxic effects that neonicotinoids can have on Apis mellifera, while few studies have evaluated the impact of these insecticides can have on the larval stage of the honeybee. From the lethal concentration (LC50) of thiamethoxam for the larvae of the Africanized honeybee, we evaluated the sublethal effects of this insecticide on morphology of the brain. After determine the LC50 (14.34 ng/mu L of diet) of thiamethoxam, larvae were exposed to a sublethal concentration of thiamethoxam equivalent to 1.43 ng/mu L by acute and subchronic exposure. Morphological and immunocytochemistry analysis of the brains of the exposed bees, showed condensed cells and early cell death in the optic lobes. Additional dose-related effects were observed on larval development. Our results show that the sublethal concentrations of thiamethoxam tested are toxic to Africanized honeybees larvae and can modulate the development and consequently could affect the maintenance and survival of the colony. These results represent the first assessment of the effects of thiamethoxam in Africanized honeybee larvae and should contribute to studies on honey bee colony decline. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 09/06701-5 - ANÁLISE MORFOLÓGICA E IMUNOCITOQUÍMICA DO CÉREBRO DE LARVAS DE ABELHAS AFRICANIZADAS Apis mellifera APÓS EXPOSIÇÃO À DOSES SUBLETAIS DE TIAMETOXAM
• Beneficiário: Daiana Antonia Tavares
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 13/09419-4 - Interação entre pesticidas e infecção por Nosema em Apis mellifera africanizada: efeitos biológicos e detecção de biomarcadores celulares
• Beneficiário: Elaine Cristina Mathias da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/50197-2 - Ação de produtos empregados no cultivo da cana-de-açúcar sobre organismos não alvos
• Beneficiário: Carmem Silvia Fontanetti Christofoletti
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático