Effect of Bacteriocins and Conditions that Mimic Food and Digestive Tract on Biofilm Formation, In Vitro Invasion of Eukaryotic Cells and Internalin Gene Expression by Listeria monocytogenes

Autor(es)

Winkelstroeter, Lizziane Kretli ^[1] ; De Martinis, Elaine C. P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo FCFRP USP, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PROBIOTICS AND ANTIMICROBIAL PROTEINS; v. 5, n. 3, p. 153-164, SEP 2013.
• Citações Web of Science: 2

Resumo

In this report, Listeria monocytogenes isolates were evaluated for their ability to form biofilms, for adhesion/invasion of eukaryotic cells and for differential expression of internalin A (inl A) gene, which is related to virulence potential. The presence of bacteriocins of lactic acid bacteria and incubation at 5 degrees C were the main factors that influenced biofilm formation by L. monocytogenes, in comparison with BHI (control). In general, adhesion and invasion of Caco-2 cells were significantly lower in low pH (4.5), in incubation at 5 degrees C and in the presence of Oxgall 0.3 %. On the other hand, two L. monocytogenes isolates (INCQS 353 and Reg 26c) showed higher invasion rates when cultivated in the presence of NaCl 5 % (P < 0.05). One L. monocytogenes isolate (H-2) showed the strongest ability to form biofilm and to invade Caco-2 cells, under selected conditions, suggesting there is a relationship between biofilm formation and virulence potential. For all isolates, expression of inl A gene was down-regulated by the presence of bacteriocins, Oxgall 0.3 %, pH 4.5 and incubation at 5 degrees C. Nonetheless, for one L. monocytogenes isolate (HU 471), expression of inl A gene was eight times higher in the presence of sucrose, indicating that food components can increase the infectiveness of L. monocytogenes. (AU)

• Processo FAPESP: 08/58300-1 - Analise de expressao genica, formacao de biofilmes e adesao/invasao a celulas caco-2 por listeria monocytogenes em diferentes condicoes encontradas no trato gastrintestinal,em alimentos e em presenca...
• Beneficiário: Lizziane Kretli Winkelströter Eller
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 09/51141-8 - Análise de expressão gênica, formação de biofilme e adesão a células Caco-2 por Listeria monocytogenes em diferentes condições encontradas em alimentos
• Beneficiário: Elaine Cristina Pereira de Martinis
• Modalidade de apoio: Auxílio à Pesquisa - Regular