Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Complete Chloroplast Genome of Tanaecium tetragonolobum: The First Bignoniaceae Plastome

Texto completo
Autor(es):
Nazareno, Alison Goncalves [1] ; Carlsen, Monica [2] ; Lohmann, Lucia Garcez [1]
Número total de Autores: 3
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Biociencias, Dept Bot, Sao Paulo, SP - Brazil
[2] Univ Missouri, Dept Biol, St Louis, MO 63121 - USA
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 10, n. 6 JUN 23 2015.
Citações Web of Science: 26
Resumo

Bignoniaceae is a Pantropical plant family that is especially abundant in the Neotropics. Members of the Bignoniaceae are diverse in many ecosystems and represent key components of the Tropical flora. Despite the ecological importance of the Bignoniaceae and all the efforts to reconstruct the phylogeny of this group, whole chloroplast genome information has not yet been reported for any members of the family. Here, we report the complete chloroplast genome sequence of Tanaecium tetragonolobum (Jacq.) L.G. Lohmann, which was reconstructed using de novo and referenced-based assembly of single-end reads generated by shotgun sequencing of total genomic DNA in an Illumina platform. The gene order and organization of the chloroplast genome of T. tetragonolobum exhibits the general structure of flowering plants, and is similar to other Lamiales chloroplast genomes. The chloroplast genome of T. tetragonolobum is a circular molecule of 153,776 base pairs (bp) with a quadripartite structure containing two single copy regions, a large single copy region (LSC, 84,612 bp) and a small single copy region (SSC, 17,586 bp) separated by inverted repeat regions (IRs, 25,789 bp). In addition, the chloroplast genome of T. tetragonolobum has 38.3% GC content and includes 121 genes, of which 86 are protein-coding, 31 are transfer RNA, and four are ribosomal RNA. The chloroplast genome of T. tetragonolobum presents a total of 47 tandem repeats and 347 simple sequence repeats (SSRs) with mononucleotides being the most common and di-, tri-, tetra-, and hexanucleotides occurring with less frequency. The results obtained here were compared to other chloroplast genomes of Lamiales available to date, providing new insight into the evolution of chloroplast genomes within Lamiales. Overall, the evolutionary rates of genes in Lamiales are lineage-, locus-, and region-specific, indicating that the evolutionary pattern of nucleotide substitution in chloroplast genomes of flowering plants is complex. The discovery of tandem repeats within T. tetragonolobum and the presence of divergent regions between chloroplast genomes of Lamiales provides the basis for the development of markers at various taxonomic levels. The newly developed markers have the potential to greatly improve the resolution of molecular phylogenies. (AU)

Processo FAPESP: 12/50260-6 - Estruturação e evolução da biota amazônica e seu ambiente: uma abordagem integrativa
Beneficiário:Lúcia Garcez Lohmann
Linha de fomento: Auxílio à Pesquisa - Programa BIOTA - Temático
Processo FAPESP: 11/50859-2 - Sistemática da tribo Bignonieae (Bignoniaceae)
Beneficiário:Lúcia Garcez Lohmann
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 13/12633-8 - Filogeografia comparada de plantas na Amazônia Central
Beneficiário:Alison Gonçalves Nazareno
Linha de fomento: Bolsas no Brasil - Pós-Doutorado