Induction, expression and characterisation of laccase genes from the marine-derived fungal strains Nigrospora sp CBMAI 1328 and Arthopyrenia sp CBMAI 1330

Autor(es)

Zambrano Passarini, Michel Rodrigo ^[1] ; Ottoni, Cristiane Angelica ^[2] ; Santos, Cledir ^{[2, 3]} ; Lima, Nelson ^{[2, 3]} ; Sette, Lara Duraes ^{[1, 4]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, CPQBA, Div Recursos Microbianos, BR-13083970 Campinas, SP - Brazil

^[2] Univ Minho, Ctr Biol Engn, CEB, P-4710057 Braga - Portugal

^[3] Univ Fed Lavras, Postgrad Programme Agr Microbiol, Lavras, MG - Brazil

^[4] UNESP, Inst Biociencias, Dept Bioquim & Microbiol, BR-13506900 Rio Claro, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: AMB EXPRESS; v. 5, MAR 11 2015.
• Citações Web of Science: 4

Resumo

The capability of the fungi Nigrospora sp. CBMAI 1328 and Arthopyrenia sp. CBMAI 1330 isolated from marine sponge to synthesise laccases (Lcc) in the presence of the inducer copper (1-10 mu M) was assessed. In a liquid culture medium supplemented with 5 mu M of copper sulphate after 5 days of incubation, Nigrospora sp. presented the highest Lcc activity (25.2 U.L-1). The effect of copper on Lcc gene expression was evaluated by reverse transcriptase polymerase chain reaction. Nigrospora sp. showed the highest gene expression of Lcc under the same conditions of Lcc synthesis. The highest Lcc expression by the Arthopyrenia sp. was detected at 96 h of incubation in absence of copper. Molecular approaches allowed the detection of Lcc isozymes and suggest the presence of at least two undescribed putative genes. Additionally, Lcc sequences from the both fungal strains clustered with other Lcc sequences from other fungi that inhabit marine environments. (AU)

• Processo FAPESP: 08/06720-7 - Caracterização da diversidade de fungos filamentosos associados a esponjas marinhas e avaliação da produção de lacase
• Beneficiário: Michel Rodrigo Zambrano Passarini
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/19486-0 - Biotecnologia marinha e Antártica: enzimas microbianas e suas aplicações
• Beneficiário: Lara Durães Sette
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/50190-2 - Investigação do potencial biotecnológico e metabólico de organismos marinhos para processos de biorremediação e produção de substâncias com atividades anti-virais, anti-leishmania e anti-inflamatória (Biodiversidade Marinha)
• Beneficiário: Roberto Gomes de Souza Berlinck
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático