Induction, expression and characterisation of laccase genes from the marine-derived fungal strains Nigrospora sp CBMAI 1328 and Arthopyrenia sp CBMAI 1330

Author(s)

Zambrano Passarini, Michel Rodrigo ^[1] ; Ottoni, Cristiane Angelica ^[2] ; Santos, Cledir ^{[2, 3]} ; Lima, Nelson ^{[2, 3]} ; Sette, Lara Duraes ^{[1, 4]}

Affiliation

^[1] Univ Estadual Campinas, CPQBA, Div Recursos Microbianos, BR-13083970 Campinas, SP - Brazil

^[2] Univ Minho, Ctr Biol Engn, CEB, P-4710057 Braga - Portugal

^[3] Univ Fed Lavras, Postgrad Programme Agr Microbiol, Lavras, MG - Brazil

^[4] UNESP, Inst Biociencias, Dept Bioquim & Microbiol, BR-13506900 Rio Claro, SP - Brazil

• Document type: Journal article
• Source: AMB EXPRESS; v. 5, MAR 11 2015.
• Web of Science Citations: 4

Abstract

The capability of the fungi Nigrospora sp. CBMAI 1328 and Arthopyrenia sp. CBMAI 1330 isolated from marine sponge to synthesise laccases (Lcc) in the presence of the inducer copper (1-10 mu M) was assessed. In a liquid culture medium supplemented with 5 mu M of copper sulphate after 5 days of incubation, Nigrospora sp. presented the highest Lcc activity (25.2 U.L-1). The effect of copper on Lcc gene expression was evaluated by reverse transcriptase polymerase chain reaction. Nigrospora sp. showed the highest gene expression of Lcc under the same conditions of Lcc synthesis. The highest Lcc expression by the Arthopyrenia sp. was detected at 96 h of incubation in absence of copper. Molecular approaches allowed the detection of Lcc isozymes and suggest the presence of at least two undescribed putative genes. Additionally, Lcc sequences from the both fungal strains clustered with other Lcc sequences from other fungi that inhabit marine environments. (AU)

• FAPESP's process: 08/06720-7 - Characterization of filamentous fungi diversity from marine sponges and evaluation of laccase production
• Grantee: Michel Rodrigo Zambrano Passarini
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/19486-0 - Marine and Antarctic biotechnology: microbial enzymes and their applications
• Grantee: Lara Durães Sette
• Support Opportunities: Regular Research Grants
• FAPESP's process: 10/50190-2 - Investigation of metabolic and biotechnological potential of marine organisms in bioremediation processes and for the production of substances with anti-viral, anti-inflammatory and anti-Leishmania activities (Marine Biodiversity)
• Grantee: Roberto Gomes de Souza Berlinck
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants