Study of global gene expression and genomic regions associated with control expression of enzymes involved in the degradation of lignocellulosic compounds by Trichoderma harzianum

• Grant number: 14/18856-1
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Effective date (Start): December 01, 2014
• Effective date (End): November 30, 2017
• Field of knowledge: Agronomical Sciences - Agronomy - Crop Science
• Principal Investigator: Anete Pereira de Souza
• Grantee: Maria Augusta Crivelente Horta
• Host Institution: Centro de Biologia Molecular e Engenharia Genética (CBMEG). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

The study of the constitution of the genome and the regulation of gene expression activities of the fungus Trichoderma harzianum provide important information about the genetic mechanisms of degradation of biomass that the fungus uses this information that could be used for other species of filamentous fungi with potential for biodegradation. Thus this project proposes the analysis of the Trichoderma harzianum genomic regions involved in the degradation of cellulose and hemicellulose, and the study of genes and genomic sequences related to regulation and gene expression of enzymes that promote degradation of lignocellulosic compounds. Previous studies from our group determined the transcriptome of the fungus under conditions of biodegradation by transcriptome sequencing, annotation of genes and determination of expression levels of genes related to degradation, especially of cellulose and hemicellulose fractions. Another study being finalized in our group ordered large genetic regions, through libraries of BACs containing the sequences responsible for the transcriptional regulation of genes related to regions responsible for biodegradation. The extent of the studies associated with the results recently obtained by our group will bring great impact on the findings of the regulation and expression of genes responsible for biodegradation in T. harzianum. Thus, the next step is the analysis and determination of the mechanisms of gene expression regulation, proposed in this project. For the study we intend to proceed with the fermentation of other strains of T. harzianum (CBMAI CBMAI 0020 and 0179), to determine the transcriptome and the exoproteoma fermentations. Then determining the expressed genes potentially involved in the degradation reactions by analysis of global transcript and analyzing the regions present in the genome of T. harzianum IOC3844 genomic structure (sequences, genes and motifs) compared to strains 0020 and CBMAI CBMAI 0179, through the annotation of the sequences of BACs. Thus, by analyzing the difference in expression between the genes and proteins in exoproteoma different strains of the transcriptome profile elements is expected to identify the sequences which may account for the difference in gene expression.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERREIRA FILHO, JAIRE ALVES; CRIVELENTE HORTA, MARIA AUGUSTA; BELOTI, LILIAN LUZIA; DOS SANTOS, CLELTON APARECIDO; DE SOUZA, ANETE PEREIRA. Carbohydrate-active enzymes in Trichoderma harzianum: a bioinformatic analysis bioprospecting for key enzymes for the biofuels industry. BMC Genomics, v. 18, OCT 12 2017. (16/19775-0, 15/09202-0, 14/18856-1)

SANTOS, CLELTON A.; ZANPHORLIN, LET-CIA M.; CRUCELLO, ALINE; TONOLI, CELISA C. C.; RULLER, ROBERTO; HORTA, MARIA A. C.; MURAKAMI, MARIO T.; DE SOUZA, ANETE PEREIRA. Crystal structure and biochemical characterization of the recombinant ThBgl, a GH1 beta-glucosidase overexpressed in Trichoderma harzianum under biomass degradation conditions. BIOTECHNOLOGY FOR BIOFUELS, v. 9, MAR 22 2016. (14/09720-9, 08/52197-4, 14/18856-1, 13/13309-0)

CRIVELENTE HORTA, MARIA AUGUSTA; FERREIRA FILHO, JAIRE ALVES; MURAD, NATALIA FARAJ; SANTOS, EIDY DE OLIVEIRA; DOS SANTOS, CLELTON APARECIDO; MENDES, JULIANO SALES; BRANDAO, MARCELO MENDES; AZZONI, SINDELIA FREITAS; DE SOUZA, ANETE PEREIRA. Network of proteins, enzymes and genes linked to biomass degradation shared by Trichoderma species. SCIENTIFIC REPORTS, v. 8, JAN 22 2018. (11/00417-3, 14/18856-1, 15/09202-0, 15/50612-8, 16/19775-0)

FERREIRA FILHO, JAIRE A.; HORTA, MARIA AUGUSTA C.; DOS SANTOS, CLELTON A.; ALMEIDA, DEBORAH A.; MURAD, NATALIA F.; MENDES, JULIANO S.; SFORCA, DANILO A.; SILVA, CLAUDIO BENICIO C.; CRUCELLO, ALINE; DE SOUZA, ANETE P.. ``Integrative genomic analysis of the bioprospection of regulators and accessory enzymes associated with cellulose degradation in a filamentous fungus (Trichoderma harzianum){''}. BMC Genomics, v. 21, n. 1, NOV 2 2020. (17/26781-0, 17/17782-2, 15/09202-0, 18/19660-4, 16/19775-0, 14/18856-1)