The study of the constitution of the genome and the regulation of gene expression activities of the fungus Trichoderma harzianum provide important information about the genetic mechanisms of degradation of biomass that the fungus uses this information that could be used for other species of filamentous fungi with potential for biodegradation. Thus this project proposes the analysis of the Trichoderma harzianum genomic regions involved in the degradation of cellulose and hemicellulose, and the study of genes and genomic sequences related to regulation and gene expression of enzymes that promote degradation of lignocellulosic compounds. Previous studies from our group determined the transcriptome of the fungus under conditions of biodegradation by transcriptome sequencing, annotation of genes and determination of expression levels of genes related to degradation, especially of cellulose and hemicellulose fractions. Another study being finalized in our group ordered large genetic regions, through libraries of BACs containing the sequences responsible for the transcriptional regulation of genes related to regions responsible for biodegradation. The extent of the studies associated with the results recently obtained by our group will bring great impact on the findings of the regulation and expression of genes responsible for biodegradation in T. harzianum. Thus, the next step is the analysis and determination of the mechanisms of gene expression regulation, proposed in this project. For the study we intend to proceed with the fermentation of other strains of T. harzianum (CBMAI CBMAI 0020 and 0179), to determine the transcriptome and the exoproteoma fermentations. Then determining the expressed genes potentially involved in the degradation reactions by analysis of global transcript and analyzing the regions present in the genome of T. harzianum IOC3844 genomic structure (sequences, genes and motifs) compared to strains 0020 and CBMAI CBMAI 0179, through the annotation of the sequences of BACs. Thus, by analyzing the difference in expression between the genes and proteins in exoproteoma different strains of the transcriptome profile elements is expected to identify the sequences which may account for the difference in gene expression.
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