Elucidation of genetic mechanisms involved in the hydrolysis of plant biomass through functional genomics

• Grant number: 18/19660-4
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2019
• End date: September 30, 2021
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Anete Pereira de Souza
• Grantee: Anete Pereira de Souza
• Host Institution: Centro de Biologia Molecular e Engenharia Genética (CBMEG). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

The genus Trichoderma is of great biotechnological importance due to the capacity that the fungi of this group present in the degradation of cellulosic substrates and the generation of free sugars, which in turn can be exploited for the production of biofuel, with special attention to bioethanol. In this context, the main objective of this project is to carry out a systematic study focusing on the functional genome of different species belonging to the genus Trichoderma, including: T. harzianum IOC-3844, T. harzianum CBMAI-0179, T. reesei CBMAI-0711 and T. atroviride CBMAI-0020, aiming to establish the genetic bases related to the efficacy of carbohydrate metabolism. For this, structural genomic data generated through the construction of BAC libraries specific to each lineage will be correlated with RNA-Seq data of fungi grown under conditions of biomass degradation in order to analyze the level of expression / regulation of the target genes . With the results of gene expression, gene co-regulatory networks will be constructed, since these evidence the regulatory relations between the different genes. Once these networks are obtained, the search for target genes will be performed in order to identify candidate genes for action of transcription factors related to carbohydrate catabolism pathways, in order to search for common mechanisms / genes shared among the species. In addition, it is intended to biochemically characterize these gene-targets with studies of protein hieroglyph expression. Thus, the present study proposes to add new information regarding the genetic mechanism related to the biomass degradation process in Trichoderma spp., Allowing the identification of key genes for the development / improvement of enzymatic cocktails for the production of bioethanol. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERREIRA FILHO, JAIRE A.; ROSOLEN, RAFAELA R.; ALMEIDA, DEBORAH A.; DE AZEVEDO, PAULO HENRIQUE C.; MOTTA, MARIA LORENZA L.; AONO, ALEXANDRE H.; DOS SANTOS, CLELTON A.; HORTA, MARIA AUGUSTA C.; DE SOUZA, ANETE P.. Trends in biological data integration for the selection of enzymes and transcription factors related to cellulose and hemicellulose degradation in fungi. 3 BIOTECH, v. 11, n. 11, NOV 2021. (15/09202-0, 19/03232-6, 20/13420-1, 18/19660-4, 20/10536-9)

ROSOLEN, RAFAELA ROSSI; AONO, ALEXANDRE HILD; ALMEIDA, DEBORAH AIRES; FERREIRA FILHO, JAIRE ALVES; HORTA, MARIA AUGUSTA CRIVELENTE; DE SOUZA, ANETE PEREIRA. Network Analysis Reveals Different Cellulose Degradation Strategies Across Trichoderma harzianum Strains Associated With XYR1 and CRE1. FRONTIERS IN GENETICS, v. 13, p. 19-pg., 2022-02-24. (18/19660-4, 20/13420-1, 15/09202-0, 19/03232-6)

LEAL MOTTA, MARIA LORENZA; FERREIRA FILHO, JAIRE ALVES; DE MELO, RICARDO RODRIGUES; ZANPHORLIN, LETICIA MARIA; DOS SANTOS, CLELTON APARECIDO; DE SOUZA, ANETE PEREIRA. A novel fungal metal-dependent alpha-l-arabinofuranosidase of family 54 glycoside hydrolase shows expanded substrate specificity. SCIENTIFIC REPORTS, v. 11, n. 1, MAY 26 2021. (17/14253-9, 15/09202-0, 18/19660-4, 16/19775-0, 19/08855-1)

ROSOLEN, RAFAELA ROSSI; HORTA, MARIA AUGUSTA CRIVELENTE; DE AZEVEDO, PAULO HENRIQUE CAMPITELI; DA SILVA, CARLA CRISTINA; SFORCA, DANILO AUGUSTO; GOLDMAN, GUSTAVO HENRIQUE; DE SOUZA, ANETE PEREIRA. Whole-genome sequencing and comparative genomic analysis of potential biotechnological strains of Trichoderma harzianum, Trichoderma atroviride, and Trichoderma reesei. Molecular Genetics and Genomics, v. 298, n. 3, p. 20-pg., 2023-04-05. (15/09202-0, 18/19660-4, 20/13420-1, 20/10536-9)

FERREIRA FILHO, JAIRE A.; HORTA, MARIA AUGUSTA C.; DOS SANTOS, CLELTON A.; ALMEIDA, DEBORAH A.; MURAD, NATALIA F.; MENDES, JULIANO S.; SFORCA, DANILO A.; SILVA, CLAUDIO BENICIO C.; CRUCELLO, ALINE; DE SOUZA, ANETE P.. "Integrative genomic analysis of the bioprospection of regulators and accessory enzymes associated with cellulose degradation in a filamentous fungus (Trichoderma harzianum)". BMC Genomics, v. 21, n. 1, p. 14-pg., 2020-11-02. (14/18856-1, 16/19775-0, 17/17782-2, 17/26781-0, 18/19660-4, 15/09202-0)

FERREIRA FILHO, JAIRE A.; HORTA, MARIA AUGUSTA C.; DOS SANTOS, CLELTON A.; ALMEIDA, DEBORAH A.; MURAD, NATALIA F.; MENDES, JULIANO S.; SFORCA, DANILO A.; SILVA, CLAUDIO BENICIO C.; CRUCELLO, ALINE; DE SOUZA, ANETE P.. ``Integrative genomic analysis of the bioprospection of regulators and accessory enzymes associated with cellulose degradation in a filamentous fungus (Trichoderma harzianum){''}. BMC Genomics, v. 21, n. 1, NOV 2 2020. (17/26781-0, 17/17782-2, 15/09202-0, 18/19660-4, 16/19775-0, 14/18856-1)