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Study of Trichoderma harzianum genomic regions associated to the control of the expression of the enzymes involved in the degradation of biomass

Grant number: 15/09202-0
Support type:Regular Research Grants
Duration: July 01, 2016 - September 30, 2018
Field of knowledge:Biological Sciences - Genetics
Principal Investigator:Anete Pereira de Souza
Grantee:Anete Pereira de Souza
Home Institution: Centro de Biologia Molecular e Engenharia Genética (CBMEG). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Assoc. researchers:Clelton Aparecido dos Santos ; Maria Augusta Crivelente Horta

Abstract

The study of the constitution of the genome and gene expression regulation of activities of the fungus Trichoderma harzianum provide important information about the genetic mechanisms of biomass degradation that the fungus uses, information that could be used for other species of filamentous fungi with potential for biodegradation. In this way it is proposed in this project the analysis of genomic regions of Trichoderma harzianum involved in cellulose and hemicellulose degradation and the study of genes and genomic sequences related to the regulation and gene expression of enzymes that promote degradation of lignocellulosic compounds. Previous studies from our group determined the fungus transcriptome in biodegradation conditions through next generation sequencing, annotation of genes and determination of gene expression levels related to degradation, especially of cellulosic and hemicellulose fractions. Group Recent results obtained by sequencing the clones BACs (Bacterial Artificial Chromosome) determined the existence of genomic regions containing groups of genes involved in the degradation of biomass as well as their likely accessory genes and their regulatory sequences responsible for its transcription. The extent of the studies associated with the results recently obtained by our group will bring great impact on the findings of the regulation and expression of genes responsible for biodegradation in T. harzianum. In this way, the next step is the analysis and determination of the regulatory mechanisms of gene expression, proposed in this project. For the study aims to carry out the fermentation of other strains of T. harzianum (CBMAI CBMAI 0020 and 0179) and Trichoderma reesei (CBMAI 711), determine the transcriptome and the exoproteoma of fermentation. Then, determine the potentially expressed genes involved in degradation reactions by analysis of the overall transcript and analyzing the genomic structure (sequences, genes and motifs) present in regions of the genome of T. harzianum IOC3844 compared with the strains CBMAI 0020, CBMAI 0179 and T. reesei. This analysis will be conducted through annotation of BAC sequences previously selected from the existing BACs library in the laboratory (for IOC3844 lineage) and two other small libraries to be built for CBMAI 0020 and CBMAI lines 0179. Thus, for analyzing the differences in expression between the genes, proteins present in exoproteoma and transcriptome profile of the different strains is expected to identify elements in the BAC sequences from the three different strains which may account for the difference in gene expression. (AU)

Articles published in Agência FAPESP Newsletter about the research grant
Modified enzyme can increase second-generation ethanol production 
Mechanisms of hydrolytic enzyme production by fungi are elucidated 
Articles published in other media outlets (27 total):
More itemsLess items
Una enzima modificada puede incrementar la producción de etanol de segunda generación 
Modified Enzyme Can Increase Second-Generation Ethanol Production 
Modified enzyme can increase second-generation ethanol production 
Brazilian researchers find Amazonian fungus that produces key enzyme for ethanol production 
Modified Enzyme Can Increase Second-Generation Ethanol Production 
Modified enzyme can increase second-generation ethanol production 
Modified enzyme can increase second-generation ethanol production 
Modified enzyme can increase second-generation ethanol production 
The modified enzyme may increase the production of second-generation ethanol 
Modified enzyme can increase second-generation ethanol production 
Modified enzyme can increase second-generation ethanol production 
Modified enzyme can increase second-generation ethanol production 
Modified enzyme can increase second-generation ethanol production 
Modified enzyme can increase second-generation ethanol production 
Modified enzyme can increase second-generation ethanol production 
Modified enzyme can increase second-generation ethanol production 
Modified enzyme can increase second-generation ethanol production 
Modified enzyme can increase second-generation ethanol production 
Modified Enzyme Can Increase Second-Generation Ethanol Production 
Modified enzyme can increase second-generation ethanol production 
Modified enzyme can increase second-generation ethanol production 
Una enzima modificada puede incrementar la producción de etanol de segunda generación 
Modifican genéticamente una enzima para que tenga una eficiencia catalítica un 300% mayor para producir etanol 
Una enzima modificada puede incrementar la producción de etanol de segunda generación 
Investigación: una enzima modificada puede incrementar la producción de etanol de segunda generación 
Una enzima modificada puede incrementar la producción de etanol de segunda generación 
Fungo aumenta em 45% produtividade do etanol 

Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SANTOS, CLELTON A.; MORAIS, MARIANA A. B.; TERRETT, OLIVER M.; LYCZAKOWSKI, JAN J.; ZANPHORLIN, LETICIA M.; FERREIRA, JAIRE A.; TONOLI, CELISA C. C.; MURAKAMI, MARIO T.; DUPREE, PAUL; SOUZA, ANETE P. An engineered GH1 ss-glucosidase displays enhanced glucose tolerance and increased sugar release from lignocellulosic materials. SCIENTIFIC REPORTS, v. 9, MAR 20 2019. Web of Science Citations: 1.
CRIVELENTE HORTA, MARIA AUGUSTA; FERREIRA FILHO, JAIRE ALVES; MURAD, NATALIA FARAJ; SANTOS, EIDY DE OLIVEIRA; DOS SANTOS, CLELTON APARECIDO; MENDES, JULIANO SALES; BRANDAO, MARCELO MENDES; AZZONI, SINDELIA FREITAS; DE SOUZA, ANETE PEREIRA. Network of proteins, enzymes and genes linked to biomass degradation shared by Trichoderma species. SCIENTIFIC REPORTS, v. 8, JAN 22 2018. Web of Science Citations: 5.
FERREIRA FILHO, JAIRE ALVES; CRIVELENTE HORTA, MARIA AUGUSTA; BELOTI, LILIAN LUZIA; DOS SANTOS, CLELTON APARECIDO; DE SOUZA, ANETE PEREIRA. Carbohydrate-active enzymes in Trichoderma harzianum: a bioinformatic analysis bioprospecting for key enzymes for the biofuels industry. BMC Genomics, v. 18, OCT 12 2017. Web of Science Citations: 4.
SANTOS, CLELTON A.; FERREIRA-FILHO, JAIRE A.; O'DONOVAN, ANTHONIA; GUPTA, VIJAI K.; TUOHY, MARIA G.; SOUZA, ANETE P. Production of a recombinant swollenin from Trichoderma harzianum in Escherichia coli and its potential synergistic role in biomass degradation. Microbial Cell Factories, v. 16, MAY 16 2017. Web of Science Citations: 3.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SOUZA, Anete Pereira de. . 2019. Doctoral Thesis - Universidade Estadual de Campinas, Instituto de Biologia.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.