Effects of Protein A in Detection of Canine Distemper Virus Through Immunosensor Construction

Autor(es)

Basso, Caroline Rodrigues ^[1] ; Sempionatto, Juliane R. ^[1] ; Tozato, Claudia C. ^[2] ; Rocha, Gustavo C. ^[1] ; Araujo, Jr., Joao Pessoa ^[2] ; Pedrosa, Valber A. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Dept Chem & Biochem, Inst Biosci, BR-18618000 Botucatu, SP - Brazil

^[2] Sao Paulo State Univ, Dept Microbiol & Immunol, Inst Biosci, BR-18618000 Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: IEEE SENSORS JOURNAL; v. 15, n. 8, p. 4677-4683, AUG 2015.
• Citações Web of Science: 1

Resumo

We have developed a detection system to canine distemper virus (CDV) by employing surface plasmon resonance (SPR) and compared the effect of oriented and random immobilization. Several parameters, such as the surface concentration of immobilized antibodies, antigen binding capacity, and the maximum surface concentration of immobilized antibody were optimized. The maximal antigen binding capacity was obtained by means of a SPR-sensor immobilized via protein A, which leads to an end-on orientation. CDV at 0.1 ng/mL can be detected using protein A. Urine analysis showed that all samples contained detectable levels of CDV, but the results revealed an improvement in sensitivity of the assay when it is specifically oriented. The described method is simple, fast, selective, and sensitive to the biorecognition element. The results emphasize how important it is to attach proteins on surfaces in such a way that their binding sites are oriented toward the solution phase. (AU)

• Processo FAPESP: 12/15666-1 - Detecção do vírus da cinomose canina por diferentes metodologias analíticas
• Beneficiário: Caroline Rodrigues Basso
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 10/07478-5 - Amplificação da respostas biocatalíticas em nanoestruturas de ouro utilizando ressonância plasmônica de superfície localizada
• Beneficiário: Valber de Albuquerque Pedrosa
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores