Immunohistochemical study of pituitary cells in wild and captive Salminus hilarii (Characiformes: Characidae) females during the annual reproductive cycle

Autor(es)

Honji, Renato Massaaki ^[1] ; Nobrega, Rafael Henrique ^[2] ; Pandolfi, Matias ^[3] ; Shimizu, Akio ^[4] ; Borella, Maria Ines ^[2] ; Moreira, Renata Guimaraes ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biociencias, Dept Fisiol, BR-05508090 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Inst Ciencias Biomed, BR-05508090 Sao Paulo, SP - Brazil

^[3] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Biodiversidad & Biol Expt, Buenos Aires, BA - Argentina

^[4] Fisheries Res Agcy, Natl Res Inst Fisheries Sci, Yokohama, Kanagawa 2368648 - Japan

• Tipo de documento: Artigo Científico
• Fonte: SPRINGERPLUS; v. 2, 2013.
• Citações Web of Science: 9

Resumo

Freshwater fish that live exclusively in rivers are at particular risk from fragmentation of the aquatic system, mainly the species that migrate upriver for reproduction. That is the case of Salminus hilarii, an important migratory species currently classified as ``almost threatened{''} in the Sao Paulo State (Brazil), facing water pollution, dam construction, riparian habitat destruction and environmental changes that are even more serious in this State. Additionally, this species show ovulation dysfunction in captivity. Our studies focused on the identification and distribution of the pituitary cell types in the adenohypophysis of S. hilarii females, including a morphometric analysis that compares pituitary cells from wild and captive broodstocks during the reproductive annual cycle. The morphology of adenohypophysial cells showed differences following the reproductive cycle and the environment. In general, optical density suggested a higher cellular activity during the previtellogenic (growth hormone) and vitellogenic (somatolactin) stages in both environments. Additionally, the nucleus/cell ratio analysis suggested that growth hormone and somatolactin cells were larger in wild than in captive females in most reproductive stages of the annual cycle. In contrast, prolactin hormone showed no variation throughout the reproductive cycle (in both environments). Morphometrical analyses related to reproduction of S. hilarii in different environmental conditions, suggest that somatolactin and growth hormone play an important role in reproduction in teleost and can be responsible for the regulation of associated processes that indirectly affect reproductive status. (AU)

• Processo FAPESP: 01/10483-1 - Caracterizacao histologica, metabolica e molecular de peixes com migracao reprodutiva bloqueada por barragens ou confinamento em viveiros de cultivo.
• Beneficiário: Renata Guimarães Moreira Whitton
• Modalidade de apoio: Auxílio à Pesquisa - Regular