Influence of Parstatin on Experimental Periodontal Disease and Repair in Rats

Autor(es)

Spolidorio, Luis Carlos ^[1] ; Ramalho Lucas, Pablo Dallari ^[1] ; Steffens, Joao Paulo ^[1] ; Bueno da Silva, Henrique Aparecido ^[2] ; Euzebio Alves, Vanessa Tubero ^[2] ; Palomari Spolidorio, Denise M. ^[1] ; Holzhausen, Marinella ^[2]

Afiliação do(s) autor(es)

^[1] State Univ Sao Paulo, Dept Physiol & Pathol, Dent Sch Araraquara, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Dent, Dept Stomatol, Div Periodont, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Periodontology; v. 85, n. 9, p. 1266-1274, SEP 2014.
• Citações Web of Science: 6

Resumo

Background: Parstatin is a 41-amino acid peptide, formed by proteolytic cleavage on activation of the protease activated receptor-1, with antiangiogenic properties. The purpose of this study is to evaluate the influence of synthetic parstatin on experimental periodontal disease and repair in rats. Methods: Ligature-induced periodontitis was established in rats and the influence of parstatin administration was assessed after 8 and 15 days for periodontal disease and 24 hours and 8 days after repair after ligature removal. Results: Parstatin administration significantly inhibited gingival myeloperoxidase activity, interleukin (IL)-1 beta, tumor necrosis factor-alpha, and IL-6 levels and led to suppression of macrophages and collagen degradation. At periodontal tissues under repair, parstatin increased the gingival levels of endostatin and decreased vascular endothelial growth factor expression and blood vessel number but did not influence histologic healing. In addition, the tomographic linear bone loss was significantly reduced at 15 days of periodontitis when the rats were treated with parstatin compared to their respective phosphate-buffered saline-treated controls. Conclusions: Parstatin suppresses the periodontal tissue breakdown followed by experimental periodontitis in rats and did not impair periodontal tissue repair, despite its antiangiogenic effect. Parstatin may represent a novel approach to modulate host response in chronic periodontal disease. (AU)

• Processo FAPESP: 10/16605-0 - Efeito da terapia periodontal sobre a expressão de receptor ativado por protease do tipo 2 (PAR-2) em pacientes com periodontite crônica.
• Beneficiário: Marinella Holzhausen Caldeira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/10715-9 - Avaliação de citocinas liberadas por plaquetas durante o desenvolvimento da doença periodontal e reparo dos tecidos periodontais de ratos tratados ou não com drogas antiplaquetárias
• Beneficiário: Luis Carlos Spolidorio
• Modalidade de apoio: Auxílio à Pesquisa - Regular