| Texto completo | |
| Autor(es): |
Andia, Denise C.
[1]
;
de Oliveira, Naila F. P.
[1]
;
Letra, Ariadne M.
[2]
;
Nociti, Jr., Francisco H.
[3]
;
Line, Sergio R. P.
[1]
;
de Souza, Ana P.
[1]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Univ Estadual Campinas, Sch Dent Piracicaba, Mol Biol Lab, Dept Morphol, Div Histol, Piracicaba, SP - Brazil
[2] Univ Pittsburgh, Sch Dent Med, Dept Oral Biol, Pittsburgh, PA - USA
[3] Univ Estadual Campinas, Sch Dent Piracicaba, Div Periodont, Dept Prosthodont & Periodont, Piracicaba, SP - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Journal of Periodontology; v. 82, n. 6, p. 893-899, JUN 2011. |
| Citações Web of Science: | 32 |
| Resumo | |
Background: The proinflammatory chemokine interleukin (IL)-8 is important in the regulation of the inflammatory response. Analyses of the single nucleotide polymorphism (SNP) reference sequence (rs) 4073 showed that the A allele upregulated IL-8 levels after stimulation with lipopolysaccharides. We investigated the association of the SNP rs4073 with chronic periodontitis. Methods: Genotyping was performed by a standard polymerase chain reaction restriction fragment length polymorphism assay in 289 genomic DNA samples of healthy control subjects and patients with chronic periodontitis; analyses were adjusted by multivariate logistic regression modeling. A real-time polymerase chain reaction performance was used to detect levels of the IL-8 mRNA. Results: The analysis pointed to a statistically significant association of chronic periodontitis with the heterozygous TA genotype (P = 0.001); the results showed an increase in the frequency of the A allele in the diseased group (36% in the control group versus 48% in the periodontitis group). The higher levels of the IL-8 mRNA were found in the periodontitis group, mainly in individuals who presented the TA genotype (P = 0.03). Conclusion: The SNP rs4073 was associated with chronic periodontitis in non-smoker Brazilian subjects because the frequency of the A allele was higher in the disease group than in the control group, and the TA genotype was associated with increased levels of IL-8 mRNA transcripts. J Periodontol 2011;82:893-899. (AU) | |
| Processo FAPESP: | 07/02488-0 - Análise de metilação no promotor dos genes da IL-8, iNOS, TIMP-2 e TIMP-3 em indivíduos fumantes e não-fumantes com periodontite crônica: associação com a expressão gênica destes genes no tecido gengival |
| Beneficiário: | Ana Paula de Souza |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |