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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Interleukin-8 Gene Promoter Polymorphism (rs4073) May Contribute to Chronic Periodontitis

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Author(s):
Andia, Denise C. [1] ; de Oliveira, Naila F. P. [1] ; Letra, Ariadne M. [2] ; Nociti, Jr., Francisco H. [3] ; Line, Sergio R. P. [1] ; de Souza, Ana P. [1]
Total Authors: 6
Affiliation:
[1] Univ Estadual Campinas, Sch Dent Piracicaba, Mol Biol Lab, Dept Morphol, Div Histol, Piracicaba, SP - Brazil
[2] Univ Pittsburgh, Sch Dent Med, Dept Oral Biol, Pittsburgh, PA - USA
[3] Univ Estadual Campinas, Sch Dent Piracicaba, Div Periodont, Dept Prosthodont & Periodont, Piracicaba, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of Periodontology; v. 82, n. 6, p. 893-899, JUN 2011.
Web of Science Citations: 32
Abstract

Background: The proinflammatory chemokine interleukin (IL)-8 is important in the regulation of the inflammatory response. Analyses of the single nucleotide polymorphism (SNP) reference sequence (rs) 4073 showed that the A allele upregulated IL-8 levels after stimulation with lipopolysaccharides. We investigated the association of the SNP rs4073 with chronic periodontitis. Methods: Genotyping was performed by a standard polymerase chain reaction restriction fragment length polymorphism assay in 289 genomic DNA samples of healthy control subjects and patients with chronic periodontitis; analyses were adjusted by multivariate logistic regression modeling. A real-time polymerase chain reaction performance was used to detect levels of the IL-8 mRNA. Results: The analysis pointed to a statistically significant association of chronic periodontitis with the heterozygous TA genotype (P = 0.001); the results showed an increase in the frequency of the A allele in the diseased group (36% in the control group versus 48% in the periodontitis group). The higher levels of the IL-8 mRNA were found in the periodontitis group, mainly in individuals who presented the TA genotype (P = 0.03). Conclusion: The SNP rs4073 was associated with chronic periodontitis in non-smoker Brazilian subjects because the frequency of the A allele was higher in the disease group than in the control group, and the TA genotype was associated with increased levels of IL-8 mRNA transcripts. J Periodontol 2011;82:893-899. (AU)

FAPESP's process: 07/02488-0 - Analysis of genes promotor methylation of IL-8, iNOS, TIMP-2, TIMP-3 in smoking and no-smoking patients with chronic periodontitis: association with genetic transcription of these genes in gingival tissue
Grantee:Ana Paula de Souza
Support Opportunities: Regular Research Grants